in vivo-jetPEI®-Gal

Molecule delivered



in vivo functional studies
Cancer therapy

Targeted organs

Improved delivery to liver

Injection routes

Hydrodynamic injection
Systemic delivery (Tail vein, intravenous, intraperitoneal injection)
Local injection

Number of transfections

100 µl of in vivo-jetPEI®-Gal delivery reagent is sufficient to perform 15 to 25 intravenous injections in mouse.


Store in vivo-jetPEI®-Gal at -20 ± 5 °C.
Expiry date is indicated in the certificate of analysis (available in "My account") and on the product.

Provided with

10% glucose solution


in vivo-jetPEI® is a powerful polymer based reagent used to deliver any kind of nucleic acid to target a wide range of organs and tissues in animals. in vivo-jetPEI® ligand-conjugated derivatives have been designed to enhance delivery to cell expressing specific receptors.
in vivo-jetPEI®-Gal is a galactose-conjugated in vivo-jetPEI® designed to enhance delivery to cells expressing galactose-specific membrane lectins, such as hepatocytes bearing the asialoglycoprotein receptor (ASGP-R or Gal/GalNAc receptor).

Ordering information

Reference NumberAmount of reagentAmount of 10% glucose solution
101000047in vivo-jetPEI®-Gal 0.1 mL10 ml


Suitable for in vivo delivery of DNA, siRNA, miRNA, oligonucleotides and mRNA

in vivo-jetPEI®-Gal is the reagent of choice to deliver any type of nucleic acid to mediate gene expression or gene silencing in various tissues, with enhanced targeting to liver.
in vivo-jetPEI®-Gal is very versatile as it is suitable for the delivery of plasmid DNA, siRNA, shRNA, miRNA, oligonucleotides and mRNA.

Easy two-step protocol

in vivo-jetPEI®-Gal is a very easy-to-use delivery reagent. The protocol consists in preparing the nucleic acid and reagent separately in 5% glucose solution, mixing them together and then, after a 15 min incubation time at room temperature, injecting the nucleic acid/ in vivo-jetPEI®-Gal complexes formed into animal (Fig. 1 ).

Fig. 1: in vivo-jetPEI-Gal – Protocol

Suitable for any animal model

in vivo-jetPEI®-Gal is ideal for nucleic acid delivery in mice. However, the protocol is so easy and versatile that it can be adapted to other species including rat.
Our delivery experts are available to adapt our protocol to your animal model and provide you with starting conditions for your application (Please contact the technical support team at support@polyplus-transfection.com).

Safe: no inflammatory response induced

in vivo-jetPEI®-Gal offers a reliable and safe alternative to viral vectors as it does not induce any significant inflammatory response.


in vivo functional studies

in vivo-jetPEI®-Gal is perfectly suited to study gene function in the liver in vivo and provides the easiest method for the validation into animals of in vitro functional studies.

Read more…

Cancer Therapy

in vivo-jetPEI®-Gal-based nucleic acid delivery can be used for tumor growth inhibition studies in the liver.

Read more…


Here is a selection of relevant references using in vivo-jetPEI®-Gal, more are available in our Polyplus-transfection Database.

Nickerson, H. D., Colledge, W. H. (2004). A LacZ-based transgenic mouse for detection of somatic gene repair events in vivo., Gene Ther 11, 1351.

Xia, Y. J., Zeng, D., Xia, L. M., Yu, F., Lin, H. H., Zhou, L., Tian, D. A., Liu, M. (2012). Role of monokine induced by interferon-gamma in liver injury induced by hepatitis B virus in mice., J Viral Hepat 19, 509-1.


Polyplus-transfection® is ISO 9001 Quality Management System accredited since 2002; this level of certification assures global customers that the supplier has established reliable and effective processes for product development, manufacturing, sales and customer support.

Each lot of in vivo-jetPEI®-Gal is validated by DNA (pCMV-Luciferase) delivery into mouse through intravenous injection. The amount of luciferase (ng) per mg of protein is indicated on the Certificate of Analysis for each lot of reagent. Moreover, absence of endotoxin is verified in every lot of in vivo-jetPEI®-Gal.


In order to download a product protocol or a certificate of analysis, please create an account on Polyplus-transfection® Portal.

Why would you need to create an account?

In this personal area you will have access to:

  • Product Protocols
  • Certificates of Analysis
  • Exclusive webinars/articles
  • And surprise features!

Create or Access your account

Other files


Order by :  
Found 8 results :
Cell Linein vitro
in vivo
Delivered MoleculeReagentResults & Citations
-in vivoDNAin vivo-jetPEI-Gal
Liu, H. et al. (2018)

Front Immunol 9, 2479
TRIM21 Restricts Coxsackievirus B3 Replication, Cardiac and Pancreatic Injury via Interacting With MAVS and Positively Regulating IRF3-Mediated Type-I Interferon Production
More details
-in vivoDNA, oligonucleotidein vivo-jetPEI-Gal
Nickerson, H. D. et al. (2004)

Gene Ther 11, 1351-7
A LacZ-based transgenic mouse for detection of somatic gene repair events in vivo
More details
-in vivomimic miRNAin vivo-jetPEI-Gal
Guan, Y. et al. (2015)

Int J Biol Sci 11, 1257-68
NF-kappaB-DICER-miRs Axis Regulates TNF-alpha Expression in Responses to Endotoxin Stress
More details
-in vivosiRNAin vivo-jetPEI-Gal
Tanida T. et al. (2020)

Novel metabolic system for lactic acid via LRPGC1/ERRγ signaling pathway
More details
-in vivosiRNAin vivo-jetPEI-Gal
Xia, Y. J. et al. (2012)

J Viral Hepat 19, 509-18
Role of monokine induced by interferon-gamma in liver injury induced by hepatitis B virus in mice
More details
H358in vivosiRNAin vivo-jetPEI-Gal
Boudria, A. et al. (2018)

Oncogene ,
VEGF165b, a splice variant of VEGF-A, promotes lung tumor progression and escape from anti-angiogenic therapies through a beta1 integrin/VEGFR autocrine loop
More details
-in vivomiRNAin vivo-jetPEI-Gal
Mansoori B. et al. (2021)

Genes (Basel) 12,
miR-34a and miR-200c Have an Additive Tumor-Suppressive Effect on Breast Cancer Cells and Patient Prognosis
More details
HeLain vitro / in vivoDNA, siRNAin vivo-jetPEI-Gal, INTERFERin
Li, M. et al. (2015)

Antiviral Res 123, 50-61
Zinc finger antiviral protein inhibits coxsackievirus B3 virus replication and protects against viral myocarditis
More details