Specifications

Product

FectoCHO® Expression system

Application

Protein / Antibody Production

Kit components

FectoCHO® CD Expression Medium
FectoPRO® Transfection reagent
FectoPRO® Expression Booster

Cell types

Suspension CHO cells such as CHO-K1, CHO-S or ExpiCHO™-S

Number of transfections

1 mL of FectoPRO® Transfection reagent is sufficient to transfect 800 ml to more than 1 liter of FectoCHO® CD Expression Medium

Storage

Store FectoPRO® and FectoCHO® CD Expression Medium at 5 °C ± 3°C. Do not freeze.
Expiry date is indicated in the certificate of analysis (available in "My account") and on the product.

Overview

The FectoCHO® Expression system, consists in the synergistic association of a novel CHO chemically defined medium, FectoCHO® CD Expression medium and a powerful transfection reagent, FectoPRO®.

FectoCHO® CD Expression medium is optimised to facilitate adaptation and cultivation of various strains of CHO cells such as CHO-K1, CHO-S and ExpiCHO™-S : extensive sequential adaptation is not required. FectoPRO®-mediated transient transfection in this chemically defined medium is optimal and therefore leads to the highest protein and antibody yields using Transient Gene Expression; with up to 4 times higher protein yields than with the competitors’ systems.

Ordering Information

Reference NumberExpression MediumTransfection ReagentExpression Booster
101000011FectoCHO® CD Medium 1 LFectoPRO® 1 mL1mL
101000003FectoCHO® CD Medium 6 x 1 L--
101000014-FectoPRO® 1 mL1mL
101000007-FectoPRO® 10 mL10mL
101000019-FectoPRO® 100 mL4x10mL
716-01LKIT1 L1 mL1 mL
716-06L6 x 1 L--
116-001-10 mL10 mL
116-010-10 mL10 mL
116-040-4 x 10 mL4 x 10 mL
301000270***NO DISPLAY*** FectoCHO

1 mL of FectoPRO® Transfection Reagent is sufficient to transfect 800 ml to more than 1 liter of FectoCHO® CD Expression Medium. Bulk quantities are available upon request.

 

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Capabilities

Outstanding protein production yields in suspension CHO cells reaching up to 4 times compared to competitors’ systems.

The combination of a chemically defined medium optimized for transient transfection and protein expression, FectoCHO® CD Expression medium, and of a highly efficient transfection reagent, FectoPRO® leads to outstanding protein production yields in various CHO cells, such as CHO-S and CHO-K1 cells.

Ideal for high density cell systems such as ExpiCHO™-S cells

FectoCHO® Expression system is particularly well suited for high density cells, such as the ExpiCHO™-S cells. FectoCHO® CD medium is able to sustain growth and viability at very high cell density for up to 10 days, while ensuring a higher transfection efficiency with FectoPRO®, leading to very high protein yields in ExpiCHO™-S cells.

Excellent CHO cell adaptation

FectoCHO® CD Expression medium allows for CHO cells to reach high cell density, while maintaining an excellent viability with short doubling time. This medium has been specifically developed to allow a quick cell adaptation, whatever the CHO cell type used.

Chemically defined kit components

FectoCHO® expression system is a 3 component kit optimized to work together: FectoCHO® CD Expression Medium, FectoPRO® Transfection Reagent and FectoPRO® Expression Booster. All of the above being chemically defined components for a convenient use in biomanufacturing processes.

All Data

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Product Documents

You have access to all the documents related to the transfection reagent.

Polyplus Database

Search for publications in our Transfection Database with Polyplus transfection reagents

Lexicon

This lexicon will help you to understand the different terms related to Polyplus-transfection®.

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Resources

Articles

Multigene expression plasmid made simple
SARS-CoV-2 coronavirus (COVID-19) transfection research highlights 
Improving expression and purification of mAb using FectoCHO® Expression System and MabXpure™.

Webinars

Webinar replay: Production of difficult-to-express proteins: strategies to increase the success rate
Webinar replay : Fast and Efficient Recombinant Protein Expression Optimization

Visuals

Poster : Improving transient CHO and HEK-293 Expression Systems with a powerful transfection solution for high protein production yields: FectoPRO®
Poster : Improving mAbs yield and quality through plasmid engineering
Poster : Advanced CHO Cell Expression System for Increased Transient Protein Production

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