FectoCHO™ Expression System
Enhanced transient protein production in CHO cells
- Outstanding protein production yields in various CHO cells
- Chemically defined kit components
- Excellent lot to lot reproducibility
- Cost-effective solution
FectoCHO™ Expression system
Protein / Antibody Production
FectoCHO™ CD Expression Medium
Suspension CHO cells such as CHO-K1, CHO-S or ExpiCHO™-S
|Number of transfections|
1 mL of FectoPRO® Transfection reagent is sufficient to transfect 800 ml to more than 1 liter of FectoCHO™ CD Expression Medium
5°C ± 3°C
VIDEO: Transient protein production with FectoCHO Expression System
The growing need in the biopharmaceutical market for various recombinant proteins and antibodies produced in a short time requires an efficient and flexible system. Transient expression is a commonly used process to face this demand but is unfortunately limited by transfection efficiency and inherent productivity, especially with CHO cells. To overcome this issue, we developed an advanced transient expression system, FectoCHO Expression system, consisting in the synergistic association of a novel CHO chemically defined medium, FectoCHO™ CD Expression medium and a powerful transfection reagent, FectoPRO®.
FectoCHO™ CD Expression medium is optimised to facilitate adaptation and cultivation of various strains of CHO cells such as CHO-K1, CHO-S and ExpiCHO™-S : extensive sequential adaptation is not required. FectoPRO®-mediated transient transfection in this chemically defined medium is optimal and therefore leads to the highest protein and antibody yields using Transient Gene Expression; with up to 4 times higher protein yields than with the competitors’ systems.
|Product||Reference Number||Expression Medium||Transfection Reagent||Expression Booster|
|FectoCHO™ Expression System||716-01LKIT||1 L||1 mL||1 mL|
|FectoCHO™ Expression System||716-06LKIT||6 x 1 L||5 x 1 mL||5 x 1 mL|
|FectoCHO™ CD Medium||716-01L||1 L||-||-|
|FectoCHO™ CD Medium||716-06L||6 x 1 L||-||-|
|FectoPRO® Reagent||116-001||-||1 mL||1 mL|
|FectoPRO® Reagent||116-010||-||10 mL||10 mL|
|FectoPRO® Reagent||116-040||-||4 x 10 mL||4 x 10 mL|
Outstanding protein production yields in suspension CHO cells
The combination of a chemically defined medium optimized for transient transfection and protein expression, FectoCHO™ CD Expression medium, and of a highly efficient transfection reagent, FectoPRO® leads to outstanding protein production yields in various CHO cells, such as CHO-S and CHO-K1 cells. (Fig. 1)
The ability of FectoCHO™ Expression medium to maintain the cells in an ideal physiological state before, during and after transfection, coupled to the extremely high transfection efficiency of FectoPRO® leads to produce amazing protein and antibody yields using Transient Gene Expression. Indeed, FectoCHO™ Expression System will boost your protein yields up to 4 times compared to competitors’ expression systems. (Fig. 2)
Ideal for high density cell systems such as ExpiCHO™-S cells
FectoCHO™ Expression system is particularly well suited for high density cells, such as the ExpiCHO™-S cells. FectoCHO™ CD medium is able to sustain growth and viability at very high cell density for up to 10 days, while ensuring a higher transfection efficiency with FectoPRO®, leading to very high protein yields in ExpiCHO™-S cells. (Fig. 3)
CHO cell adaptation
FectoCHO™ CD Expression medium allows for CHO cells to reach high cell density, while maintaining an excellent viability with short doubling time. This medium has been specifically developed to allow an easy and quick cell adaptation, whatever the CHO cell type used. (Fig. 4) The cells can be directly thawed in the FectoCHO™ CD Expression medium, then transfected after a few passages to reach maximal protein expression yields. Sequential adaptation is not required, hence leading to a hassle-free and time-saving cell adaptation.
Suspension CHO cells can also be directly switched from their previous medium to FectoCHO™ CD Medium without sequential adaptation, with no impact on cell growth and viability.
Chemically defined kit components
FectoCHO™ expression system is a 3 component kit optimized to work together:
- FectoCHO™ CD Expression Medium, a chemically defined cell culture medium optimized for transient gene expression
- FectoPRO® Transfection Reagent to produce amazing protein yields using low DNA amount
- FectoPRO® Expression Booster that enhances gene expression to boost the protein production
Excellent lot to lot reproducibility
FectoCHO™ Expression System components are manufactured and formulated using a highly controlled production process. This ensures highly reproducible results from experiment to experiment, hence data that can be easily repeated and protein productions with similar yields. (Fig. 5)
FectoCHO™ Expression system is perfectly suited for protein and antibody production using transient gene expression in various CHO cell lines, such as CHO-K1, CHO-S and ExpiCHO™-S cells. The chemically defined components of the kit have been optimized to provide synergistic effects and outstanding production yields.
“I have used FectoPRO to do transient transfection on Expi293 cells for multiple constructs. My construct is usually followed by P2A and mCherry. The transfection efficiency is good, >90% as visualized by mCherry signal 48hours post transfection. Cell viability is also good, usually >95% 48 hours post transfection. Protein yield is high, ranging from 1mg to 30mg per liter depending on constructs. I have recommended FectoPRO to several friends. Thank you for the great reagent.” – Jing, Springer Lab, Boston Children’s Hospital
“I have used Polyplus FectoPRO many times for both large scale transient transfection and for the establishement of stable cell lines. The reagent is easy to use, and I have received very high yields (>130 mg/L) when expressing IgG constructs in Expi293 cells. I can’t recommend this reagent enough. – Jordan, Springer Lab, Boston Children’s Hospital
FectoCHO™ Expression system is compliant with biomanufacturing guidelines. Indeed, FectoPRO®, FectoPRO® booster and FectoCHO™ CD Expression medium are guaranteed free of animal-origin components, and are chemically defined. Systematic lot management and release testing is performed for each lot produced. Furthermore, each component of the FectoCHO® Expression System undergoes advanced quality controls such as protein productivity, cell viability and complete sterility.
In addition, Polyplus-transfection® is ISO 9001 Quality Management System accredited since 2002; this level of certification assures global customers that the supplier has established reliable and effective processes for product development, manufacturing, sales and customer support.