Try to win an iPad by filling out this form set up for PEPtalk attendees only!
* These fields are required.
Improving protein production in transient CHO and HEK-293 Expression systems with FectoPRO®, a high yield and versatile transfection solution
The development process for producing a biotherapeutic protein usually begins with generating a high-performing stable cell line which can be used for manufacturing. As this step takes a lot of time, transient transfection offers a great alternative to quickly produce milligram to gram quantities of recombinant proteins and antibodies. A various number of culture media are available for performing transient protein production in both CHO and HEK cells but the limiting factor is often the transfection reagent. That’s why we have developed a novel technologically advanced transfection solution called FectoPRO®. Here we show that FectoPRO® outperforms currently available PEI-based and lipid-based transfection reagents in all the transient expression systems tested, offering great transfection efficiency and amazing protein yields.
Development of an optimized PEI-based transfection reagent for the production of clinical grade viral vectors: PEIpro®
Gene- and cell therapy-based medicine are experiencing resurgence due to the introduction of “next generation” transfer viral vectors, which have demonstrated improved safety and efficacy. Adeno Associated Virus (AAV) and Lentivirus are very commonly used in therapeutics and often produced using PEI-mediated transient transfection in HEK-293 or HEK-293T cells. PEIpro® and its high-quality counterpart PEIpro®-HQ are particularly well suited for high titer virus production. Both reagents are manufactured under a well-controled and reproducible process and are free of components of animal-origin. Here we show that they have been selected for their high transfection efficiency using low DNA amount. Moreover, they undergo stringent quality controls, making PEIpro® and PEIpro®-HQ the unique PEI-based transfection reagents suitable for use in process development and in cGMP biomanufacturing, respectively.
Julien Depollier and Géraldine Guerin-Peyrou attended this meeting to discuss how to improve your Bioproduction system. We was happy to answer all your questions regarding transfection and to present you relevant products adapted to your experiments.
Business Director Americas
Technical Support Director
Our solutions for Bioproduction
FectoPRO® Transfection kit is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount (< 1 µg/ml of cell culture).
PEIpro® is a DNA transfection reagent for production of viruses, recombinant proteins and antibodies either in adherent cells in presence of serum or in suspension HEK-293 cells grown in low serum or serum-free media.
PEIpro®-HQ is a highly qualified grade of PEIpro® reagent, especially developed for the production of clinical batches of viral vectors or recombinant proteins and antibodies. The PEIpro®-HQ grade is supplied with appropriate Quality Controls and documentation allowing its use as a qualified raw material in GMP processes for the manufacturing of clinical batches of therapeutic products.
The convention took place at Hilton San Diego Bayfront:
1 Park Blvd, San Diego, CA, United States
For more information, please have a look at the agenda and register now to meet the delivery experts. Two Posters will be presented focusing on FectoPRO® (Protein production) and PEIpro® (Virus production).
Join us on the social network
We created a dedicated event on Facebook to allow you to discuss with other scientists about the PepTalk meeting or about your transfection experiments.
If you want to talk about this event (Facebook, Twitter, Google+, etc…), don’t forget to use the #PTK18 and follow the PepTalk news.