Meet our team during the Protein & Antibody Engineering summit in Lisbon (Portugal), November 13-17th, 2017.
The convention will take place at EPIC SANA Lisboa Hotel:
Av. Eng. Duarte Pacheco 15, 1070-100 Lisboa, Portugal
For more information, please have a look at the agenda and register now to meet the delivery experts. Our team will present two Posters focusing on FectoPRO® & PEIpro®, our transfection reagent especially designed for Bioproduction. You can view our posters during the Poster Session B: Wednesday-Thursday (morning) (4B : Optimising Expression Platforms).
FectoPRO®: Improving transient CHO and HEK-293 Expression systems with a powerful transfection solution for high protein production yields.
The development process for producing a biotherapeutic protein usually begins with generating a high-performing stable cell line which can be used for manufacturing. As this step takes a lot of time, transient transfection offers a great alternative to quickly produce milligram to gram quantities of recombinant proteins and antibodies. A various number culture medium are available for performing transient protein production in both CHO and HEK cells but the limiting factor is often the transfection reagent. That’s why we have developed a novel technologically advanced transfection solution called FectoPRO®. Here we show that FectoPRO® outperforms currently available PEI-based and lipid-based transfection reagents in all the transient expression systems tested, offering great transfection efficiency and amazing protein yields.
Production of clinical grade viral vectors using PEIpro®, an optimized PEI-based transfection reagent
Gene- and cell therapy-based medicine are experiencing a resurgence following the introduction of “next generation” transfer viral vectors, which have demonstrated improved safety and efficacy. Adeno Associated Virus (AAV) and Lentivirus are often produced using PEI-mediated transient transfection of HEK-293 or HEK-293T cells. PEIpro® and its high-quality counterpart PEIpro®-HQ are particularly well suited for high virus titer yields in both adherent and suspension cells. Both reagents are animal-free, and manufactured under a well-controlled and reproducible process. Accordingly, they undergo stringent quality controls, making PEIpro® and PEIpro®-HQ the unique PEI-based transfection reagents suitable for their use in process development and in cGMP biomanufacturing, respectively.
Meet Guillaume Freund & Jonathan Havard to discuss how to improve your Bioproduction system. We will be happy to answer all your questions regarding transfection and to present you relevant products adapted to your experiments.
Guillaume Freund, PhD
Technical Support Specialist
FectoPRO® Transfection kit is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount (< 1 µg/ml of cell culture).
PEIpro® is a DNA transfection reagent for production of viruses, recombinant proteins and antibodies either in adherent cells in presence of serum or in suspension HEK-293 cells grown in low serum or serum-free media.
PEIpro®-HQ is a highly qualified grade of PEIpro® reagent, especially developed for the production of clinical batches of viral vectors or recombinant proteins and antibodies. The PEIpro®-HQ grade is supplied with appropriate Quality Controls and documentation allowing its use as a qualified raw material in GMP processes for the manufacturing of clinical batches of therapeutic products.
Join us on the social network
We created a dedicated event on Facebook to allow you to discuss with other scientists about the PEGS meeting or about your transfection experiments.
If you want to talk about this event (Facebook, Twitter, Google+, etc…), don’t forget to use the #PEGSEurope and follow the PEGS news.