Meet our team at the booth #514 during Peptalk USA, in San Diego (USA), January 20-24, 2020.
Our solutions for both protein and virus production
FectoPRO® Transfection kit is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount (< 1 µg/ml of cell culture).
FectoCHO™ Expression system is a 3 component kit optimized to work together that contains FectoCHO™ CD Medium, FectoPRO® Transfection Reagent and FectoPRO® Expression Booster. FectoCHO™ Expression System has been developed to reach the highest protein production ever seen in CHO cell lines.
PEIpro® is a DNA transfection reagent for production of viruses, recombinant proteins and antibodies either in adherent cells in presence of serum or in suspension HEK-293 cells grown in low serum or serum-free media.
PEIpro®-HQ is a highly qualified grade of PEIpro® DNA transfection reagent. Process development are guaranteed to be seamlessly applicable with PEIpro®-HQ for manufacturing of viral vectors for pre-clinical and early phase clinical trial.
PEIpro®-GMP (NEW) is the highest quality grade PEI and the only transfection reagent available on the market supplied in a bag, ideal for GMP-compliant closed system manufacturing of clinical grade therapeutic viral vector,
Advanced CHO Cell Expression System for Increased Transient Protein Production
The growing need in the biopharmaceutical market for various recombinant proteins and antibodies produced in a short time requires an efficient and flexible system. Transient expression is a commonly used process to face this demand but is unfortunately limited by transfection efficiency and inherent productivity, especially with CHO cells. To overcome this issue, we developed an advanced transient expression system consisting in the synergistic association of a novel CHO chemically defined medium and a powerful transfection reagent. First, we show that this innovative medium allows easy cultivation of various strains of CHO cells without the need of an extensive sequential adaptation. In a second time, through the protocol optimization of a technologically advanced transfection solution, we demonstrate a major increase in our recombinant proteins yields.
Addressing Large-Scale Therapeutic Virus Production Using High Quality Grade PEI-based Transfection Reagents
Gene and cell therapy-based medicines are experiencing resurgence due to the introduction of “next generation” transfer viral vectors, which have demonstrated improved safety and efficacy. Adeno Associated Virus (AAV) and Lentivirus are very commonly used in therapeutics and often produced using PEI-mediated transient transfection in HEK-293 or HEK-293T cells. The critical raw materials needed for cGMP vector production must be sourced from approved suppliers and should have gone through a rigorous testing program to reduce the risk of introducing adventitious agents into the production process. Polyplus-transfection now provides PEIpro®, the unique PEI-based transfection reagents available in different quality grades, allowing a seamless transition from process development with PEIpro®-HQ to cGMP biomanufacturing with PEIpro®-GMP.
Meet Mayra to discuss on how to improve your process of Bioproduction. We will be happy to answer all your questions regarding transfection and to present relevant products adapted to protein or virus production.
The convention took place at Hilton San Diego Bayfront:
1 Park Blvd, San Diego, CA, United States