The growing need for various recombinant antibodies and other proteins in the biotechnology and biopharma sectors requires an efficient and flexible bioproduction system. “Transient expression” is a technology commonly use to introduce nucleic acids into production cell line. Current available transfection solutions are limited due to insufficient transfection efficiency resulting in low protein productivity, especially when applied to CHO (Chinese Hamster Ovary) cells.
“To overcome this issue, we have developed an advanced transient expression system, the FectoCHO™ Expression System,” stated Dr. Patrick Erbacher, CSO of Polyplus-transfection. “Our new system shows amazing protein production yields in various CHO cells.”
High productivity – even at small DNA amounts
The FectoCHO™ Expression System consists of three synergistically acting components:
- FectoCHO™ CD Expression Medium
- FectoPRO® Transfection Reagent
- FectoPRO® Expression Booster
The FectoCHO™ CD Expression Medium is a chemically defined cell culture medium, optimized for transient gene expression. It enables fast, effortless adaptation and cultivation of various strains of CHO cells, such as CHO-K1, CHO-S and ExpiCHO™-S. The FectoPRO® Transfection Reagent, when combined with the expression medium, achieves amazing protein yields, also for small amounts of DNA transfected. The FectoPRO® Expression Booster enhances gene expression levels and boosts protein production in various CHO cells. The yields are up to four times higher compared to competitors’ expression systems.
FectoCHO™ Expression System components are manufactured and formulated using a highly controlled production process. This ensures excellent lot-to-lot reproducibility – for greater reliability and higher quality in bioproduction. “Increased productivity and excellent reproducibility make our new expression system an affordable and reliable solution for bioproduction processes,” said Patrick. “The system is perfectly suited to protein and antibody production – even in therapeutic applications.”
Polyplus-transfection SA is a biotechnology company founded in 2001 that develops, markets and sells innovative solutions for the in vivo, in vitro and ex vivo delivery of nucleic acids in research, bioproduction and therapeutics. Located close to the University of Strasbourg in Eastern France, Polyplus-transfection has been ISO 9001-certified since 2002 and supplies its proprietary range of reagents for the transfection of genes and oligonucleotides. Polyplus reagents are involved in a growing number of clinical trials worldwide. In addition, Polyplus-transfection holds a broad estate of patents and licenses in the field of nucleic acid delivery.
The synergistic combination of FectoPRO® reagent and FectoCHO™ CD medium outperforms its main competitor in CHO-K1 cells. © Polyplus-transfection SA
FectoCHO™ expression system outperforms ExpiCHO™ expression system in terms of protein production kinetics in ExpiCHO-S cells. © Polyplus-transfection SA
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