Home > Troubleshooting Back Contact details Contact Name * Company / Institution * Email * Tested product * jetOPTIMUSjetPRIMEjetCRISPRin vivo-jetRNAjetPEIjetMESSENGERINTERFERinother, please specify in "Comments" Transfected biomolecule * plasmid DNAsiRNAmRNADNA & siRNA cotransfectionshRNA plasmidoligonucleotideRNP (Cas9 Protein + gRNA)protein/peptidemiRNA plasmidmiRNA oligosantimiRother Cells * Transfection efficiency and/or silencing efficiency DNA amount per well/plate or siRNA concentration Which plate format did you use? 384 well plate96-well plate24-well plate12-well plate6-well plate / 3.5 cm dish6 cm dish / 25 cm2 flask10 cm dish / 75 cm2 flask14 cm dish / 175 cm2 flaskCellSTACK / Cell factoryShaker flaskWaveBioreactorOther Did you try to optimize the transfection conditions? No YesPlease specify in "Comments" Did you compare Polyplus reagent with other products? Which ones? Which reagent did you prefer and why? Method to assess transfection or gene silencing Timepoint of analysis Did you observe toxicity? No Yes Number of cells seeded or % confluency at the time of transfection Volume of transfection mix prepared Buffer used to prepare the transfection mix Volume of reagent used Volume of transfection mix added per well Medium used during transfection Volume of medium per well, before adding the transfection mix Comments: By clicking Send, you agree to our Terms. Learn how we collect, use and share your data in our Data Policy and how we use cookies and similar technology in our privacy policy. Captcha * : -- Fields with * are required. --
