GMP AAV manufacturing process guidelines using transient transfection in a 2000 L bioreactor

Download infographic GMP AAV manufacturing process guidelines using transient transfection in a 2000 L bioreactor

Transient transfection is used as the gold standard method for AAV-based gene therapies because it brings key advantages with fastest process development and highest productivity.

Designing a cGMP compliant AAV manufacturing process using large scale transfection is now achievable using FectoVIR^®-AAV transfection reagent developped to resolve volume handling and time constraints that come with performing transfection at large scale, in addition to boosting AAV production yields.

In this infography, we share tips on critical steps to achieve end-to-end AAV manufacturing process at the 2000L bioreactor scale using FectoVIR^®-AAV. From raw material supply through USP/DSP manufacturing down to analytics, we review key process stages and provide guidance for steps that need particular attention to.

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