Summary

Meet our Team by visiting us, booth #82 and listen to our talk during the Cell & Gene Therapy stream at BioProcess International Europe 2022!

Our team

 Jean-Thomas ISSENHUTH 
 Key Account Manager 
About Jean-Thomas ISSENHUTH
Key Account Manager
Aucune information.
 Jelena Vjetrovic 
 Key Account Manager 
About Jelena Vjetrovic
Key Account Manager
Aucune information.
 Guillaume Freund 
 Scientific Support Manager 
About Guillaume Freund
Scientific Support Manager
Aucune information.

Talk

A next-generation transfection reagent for large scale rAAV manufacturing.

The number of ATMP therapeutic-based medicines for inherited genetic disorders is in constant growth, with a global 32% increase in new clinical trials in the last 4 years. ATMPs have demonstrated their success with already more than ten approved for commercialization. The success of AAV as the most promising viral vector for gene therapy is due to low immunogenicity, broad tropism and non-integrating properties. One major challenge for translation of promising research to clinical development is the manufacture of sufficient quantities of AAV. Transient transfection of suspension cells is the most commonly used production platform, as it offers significant flexibility for cell and gene therapy development. However, this method shows some limitations in large scale bioreactors: inadequate transfection protocol, reduced transfection efficiency and lower productivity. To address this concern, we present data on the novel transfection reagent showing: increased AAV titers, improved transfection protocol for large scale bioreactors and reproducibility of viral titers at different production scale. The aforementioned optimized parameters make this novel transfection reagent ideal for cell and gene therapy developers by combining the flexibility of transient transfection with scalability and speed to market.

Presented by Guillaume Freund, Ph.D, Scientific Support Manager at Polyplus-transfection®.
Tuesday, May 17th at 02:45 pm- 03:00 pm CET

 

Poster

Next-Generation Transfection Reagent for Large Scale AAV Manufacturing

Mathieu Porte, Mégane Denu, Jonathan Havard, Marine Ricordel, Coralie Stritt, Yann Philipson, Malik Hellal, Patrick Erbacher

 

Our solutions for viral vector production

FectoVIR®-AAV

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