Fast And Efficient DNA Transfection In Hard-to-transfect Cells
Author: V. Toussaint-Moreau, M. Denu, T. Benchimol, F. Prémartin, M. Porte, M. Dumont, G. Guerin-Peyrou, M. Hellal, Y. Philipson, F. Stock, P. Erbacher;
Polyplus-transfection, ILLKIRCH GRAFFENSTADEN, FRANCE.
DNA transfection remains challenging for scientists working with primary cells, stem cells and particular cell lines. This is mainly due to the fragility and slow-dividing rates of these cells. Considering the limiting steps in these hard-to-transfect cell types and based on our knowledge and expertise in transfection, we screened a proprietary chemical compounds library. Lead molecules were selected based on their superior transfection efficiencies while maintaining excellent cell viability in different hard to transfect cell types, leading to the development of a novel DNA transfection reagent, jetOPTIMUS®. This reagent improves cellular uptake and endosomal escape of DNA resulting in higher gene expression in cells. jetOPTIMUS® combines higher transfection efficiency and lower toxicity compared to other commercially available delivery solutions.
Our solutions for transfection
NEW: in vivo-jetRNA® transfection reagent specifically developed to deliver mRNA in vivo. This reagent can be used to target multiple organs, by using systemic or local injection routes, in various animal models.
in vivo-jetPEI® is a powerful polymer based reagent used to deliver any nucleic acid to any animal model. in vivo-jetPEI® forms stable complexes with the nucleic acid, protecting it from degradation and thus facilitating in vivo delivery. With a proven track record (over 200 Publications), in vivo-jetPEI® has already been used to target a wide range of organs using various administration routes.
jetMESSENGER® is a new mRNA transfection reagent designed to provide high transfection efficiency in hard-to-transfect cells such as primary cells, neurons, suspension cells and various cancer cell lines. mRNA transfection is as easy as DNA transfection, with the advantage that mRNA does not need to reach the cell nucleus for expression and does not require cell division for efficient gene expression. Hence, cells that are slow dividing or that have developed specific mechanisms to protect their genome can finally be used for gene expression.
jetOPTIMUS® is a powerful transfection reagent that improves cellular uptake and endosomal escape of DNA in adherent cells (even in hard-to-transfect cells) resulting in higher transfection efficiency.
jetPRIME® is a powerful and versatile DNA and siRNA transfection reagent for day-to-day experiments that leads to efficient and reliable scientific results. jetPRIME® ensures high DNA transfection efficiency and excellent gene silencing in a variety of adherent cells. jetPRIME® is also ideal for DNA/siRNA co-transfection or co-delivery of several plasmids. Furthermore, our jetPRIME® reagent is very gentle on cells since it requires low amounts of reagent and nucleic acid during transfection.
INTERFERin® provides very high silencing efficiency already at 1 nM siRNA and can be used in more in a wide variety of adherent and suspension cells. Using low concentration of siRNA avoids off-target effects, and its gentle mode of action ensure more robust data and excellent cell viability. Easy to use thanks to its compatibility with serum and antibiotics, INTERFERin® is also perfectly suited for transfection of miRNA and other oligonucleotides like pre-miRNA, mimic miRNA, antimiR….
Please contact our technical support to discuss about your experiment.
Meet Brian and Justin to discuss how to improve your transfection experiment on stem cells and cancer cells. We will be happy to answer all your questions regarding transfection and to present you relevant products adapted to your research.
The convention will take place at the Walter E. Washington Convention Center
801 Mt Vernon Pl NW, Washington, DC 20001, United States.