Presenters:

Talk : Plasmid engineering technology from Polyplus: How to fast track your rmAbs Discovery

Time : Wednesday June 23, 2023 : 11:00 AM – 11:10 AM CET

Summary of presentation : Early stage of recombinant monoclonal antibodies requires to assess as many as possible candidates in the minimal time as possible. Two bottlenecks can be considered: 1- the ability of generating dozens or hundreds of molecular constructs (plasmids) based on rational design; 2- the yield of expression of antibodies in small scale culture, to obtain rapidly quantity enough to conduct characterization. Generating multiple plasmids in parallel is by essence the benefit brought by the e-Zyvec’s DNA assembly technology. Here we show case how we used this technology to design and compare several plasmid layouts and identify those that will ensure the best yields in small scale culture.

Poster : Improving mAbs yield and quality through plasmid engineering

By :  Vianey DELEPLACE, Narciso COSTA, Léa KENGNE KAMKUI, Marie HAUTIERE, Didier BOQUE , Amaury HERBET, Sylvain JULIEN

Recombinant monoclonal antibodies (rmAbs) will become part of the therapeutical arsenal for numerous pathologies, The generalization of their usage will be driven by faster development program and lower production costs, both criteria being directly dependent on the productivity of our bioprocesses. During the past decades impressive progresses have been made regarding the bioproduction equipment’s, cell models and purification processes. The improvement of the biomolecular components, that are the plasmids, has somewhat lagged behind such developments. With the booming of molecular technologies of DNA assembly methods, including the proprietary e-Zyvec® method from Polyplus®, we have new opportunities to explore the potential of plasmid engineering as another leverage to increase yield and quality of rmAbs bioproduction.

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