Citation
- Authors: Zajac, O., Raingeaud, J., Libanje, F., Lefebvre, C., Sabino, D., Martins, I., Roy, P., Benatar, C., Canet-Jourdan, C., Azorin, P., Polrot, M., Gonin, P., Benbarche, S., Souquere, S., Pierron, G., Nowak, D., Bigot, L., Ducreux, M., Malka, D., Lobry, C., Scoazec, J. Y., Eveno, C., Pocard, M., Perfettini, J. L., Elias, D., Dartigues, P., Goere, D., Jaulin, F.
- Year: 2018
- Journal: Nat Cell Biol 20 296-306
- Applications: in vitro / DNA, shRNA plasmid / jetPRIME
- Cell type: HEK-293T
Description: Human embryonic kidney Fibroblast
Known as: HEK293T, 293T
Method
Lentivirus production
Abstract
Metastases account for 90% of cancer-related deaths; thus, it is vital to understand the biology of tumour dissemination. Here, we collected and monitored >50 patient specimens ex vivo to investigate the cell biology of colorectal cancer (CRC) metastatic spread to the peritoneum. This reveals an unpredicted mode of dissemination. Large clusters of cancer epithelial cells displaying a robust outward apical pole, which we termed tumour spheres with inverted polarity (TSIPs), were observed throughout the process of dissemination. TSIPs form and propagate through the collective apical budding of hypermethylated CRCs downstream of canonical and non-canonical transforming growth factor-beta signalling. TSIPs maintain their apical-out topology and use actomyosin contractility to collectively invade three-dimensional extracellular matrices. TSIPs invade paired patient peritoneum explants, initiate metastases in mice xenograft models and correlate with adverse patient prognosis. Thus, despite their epithelial architecture and inverted topology TSIPs seem to drive the metastatic spread of hypermethylated CRCs.