Citation
- Authors: Kress, E., Schwager, F., Holtackers, R., Seiler, J., Prodon, F., Zanin, E., Eiteneuer, A., Toya, M., Sugimoto, A., Meyer, H., Meraldi, P., Gotta, M.
- Year: 2013
- Journal: J Cell Biol 201 559-75
- Applications: in vitro / DNA / jetPEI
- Cell type: HeLa Kyoto
Abstract
Coordination of cell cycle events in space and time is crucial to achieve a successful cell division. Here, we demonstrate that UBXN-2, a substrate adaptor of the AAA ATPase Cdc48/p97, is required to coordinate centrosome maturation timing with mitosis. In UBXN-2-depleted Caenorhabditis elegans embryos, centrosomes recruited more AIR-1 (Aurora A), matured precociously, and alignment of the mitotic spindle with the axis of polarity was impaired. UBXN-2 and CDC-48 coimmunoprecipitated with AIR-1 and the spindle alignment defect was partially rescued by co-depleting AIR-1, indicating that UBXN-2 controls these processes via AIR-1. Similarly, depletion in human cells of the UBXN-2 orthologues p37/p47 resulted in an accumulation of Aurora A at centrosomes and a delay in centrosome separation. The latter defect was also rescued by inhibiting Aurora A. We therefore postulate that the role of this adaptor in cell cycle regulation is conserved.