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Citation

  • Authors: Jung, S. K., Kim, J. E., Lee, S. Y., Lee, M. H., Byun, S., Kim, Y. A., Lim, T. G., Reddy, K., Huang, Z., Bode, A. M., Lee, H. J., Lee, K. W., Dong, Z.
  • Year: 2014
  • Journal: Carcinogenesis
  • Applications: in vitro / DNA / jetPEI
  • Cell type: JB6

Abstract

The identification of primary molecular targets of cancer-preventive phytochemicals is essential for a comprehensive understanding of their mechanism of action. In the present study, we investigated the chemopreventive effects and molecular targets of acacetin, a flavonoid found in Robinia p seudoacacia, also known as black locust. Acacetin treatment significantly suppressed epidermal growth factor (EGF)-induced cell transformation. Immunoblot analysis revealed that acacetin attenuated EGF-induced phosphorylation of Akt and p70(S6K), which are downstream effectors of phosphatidylinositol 3-kinase (PI3-K). An immunoprecipitation kinase assay of PI3-K and pull-down assay results demonstrated that acacetin substantially inhibits PI3-K activity by direct physical binding. Acacetin exhibited stronger inhibitory effects against anchorage-dependent and -independent cell growth in cells expressing higher PI3-K activity compared with those exhibiting relatively low PI3-K activity. Binding assay data combined with computational modeling suggest that acacetin binds in an adenosine triphosphate (ATP)-competitive manner with the p110