Citation

  • Authors: Ni, J., Nolte, B., Arnold, A., Fournier, P., Schirrmacher, V.
  • Year: 2009
  • Journal: Vaccine 27 5480-7
  • Applications: in vitro / DNA / jetPEI-Macrophage
  • Cell type: RAW 264.7
    Description: Mouse monocytes/macrophages
    Known as: RAW

Abstract

We describe for the first time a short sequence of the CD11c promoter (700 bp, named as CD11cS) which induces selective antigen expression in dendritic cells (DCs). It showed a stronger promoter activity than the hitherto used long CD11c promoter (5.5 kb, named as CD11cL), which had been reported inefficient to induce immune responses. After application to the ear pinna and electroporation (EP), CD11cS based DNA vaccines induced specific B- and T-cell responses, including IFN-gamma secretion. Such vaccines achieved induction of anti-tumor immunity comparable in strength to vaccines having the strong tissue non-specific CMV promoter, in both prophylactic and therapeutic settings of mouse tumor models. This short CD11c promoter appears to be a safe and a practical tool for DC-specific gene targeting and for vaccination.

Pubmed