• Authors: Ebisawa, T., Numazawa, K., Shimada, H., Izutsu, H., Sasaki, T., Kato, N., Tokunaga, K., Mori, A., Honma, K., Honma, S., Shibata, S.
  • Year: 2010
  • Journal: Neurosci Res 66 223-7
  • Applications: in vitro / DNA / jetPEI-Macrophage
  • Cell type: Human peripheral blood mononuclear cells


Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmicity as the suprachiasmatic nucleus-the center of the biological clock. We tried to study human circadian rhythm using cultured peripheral blood mononuclear cells (PBMCs) obtained from a single collection of venous blood. Activated human PBMCs showed self-sustained circadian rhythm of clock gene expression, which indicates that they are useful for investigating human endogenous circadian rhythm.