- Authors: Ebisawa, T., Numazawa, K., Shimada, H., Izutsu, H., Sasaki, T., Kato, N., Tokunaga, K., Mori, A., Honma, K., Honma, S., Shibata, S.
- Year: 2010
- Journal: Neurosci Res 66 223-7
- Applications: in vitro / DNA / jetPEI-Macrophage
- Cell type: Human peripheral blood mononuclear cells
Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmicity as the suprachiasmatic nucleus-the center of the biological clock. We tried to study human circadian rhythm using cultured peripheral blood mononuclear cells (PBMCs) obtained from a single collection of venous blood. Activated human PBMCs showed self-sustained circadian rhythm of clock gene expression, which indicates that they are useful for investigating human endogenous circadian rhythm.