Citation
- Authors: Hao WY. et al.
- Year: 2022
- Journal: Biochem Biophys Res Commun 616 76-81
- Applications: in vitro / in vivo / siRNA / in vivo-jetPEI, INTERFERin
- Cell type: Bone Marrow-Derived Macrophages (BMDMs)
Method
Small interfering RNAs were purchased from Santa Cruz and were transfected into cells with INTERFERin (Polyplus) at 20 nM in vitro or in vivo-jetPEI (Polyplus) for in vivo knockdown according to the manufacturer's instructions.
Abstract
N6-methyladenosine (m6A) modification of mRNAs is involved in multiple essential biological processes, dynamically regulated by m6A "writers", "erasers", and "readers". Yet, the detailed functional roles of RNA m6A reader proteins, such as YTHDFs, are largely unknown. Herein we show that YTHDF1 promotes pro-inflammatory IL-1β production in macrophages during bacterial infections. YTHDF1 overexpression promotes NLRP3 translation. In vivo knockdown of YTHDF1 facilitates survival in a mouse model of sepsis. Thus, YTHDF1 participates in inflammatory responses and subsequent injuries, serving as a new potential therapeutic target in clinical treatment of inflammatory diseases.