- Authors: Lang YD. et al.
- Year: 2019
- Journal: Nat Commun 10 5716
- Applications: in vivo / DNA / in vivo-jetPEI
ADN/PEI/Buffer: Vortex 10 sec and incubated 15 min 37°C before injection. Each injection : 100µl glucose saline solution + 100µl transfection solution
Hepatocellular carcinoma (HCC) is one of the most lethal cancers worldwide due to metastasis. Paraspeckle component 1 (PSPC1) upregulation has been identified as an HCC pro-metastatic activator associated with poor patient prognosis, but with a lack of targeting strategy. Here, we report that PSPC1, a nuclear substrate of PTK6, sequesters PTK6 in the nucleus and loses its metastasis driving capability. Conversely, PSPC1 upregulation or PSPC1-Y523F mutation promotes epithelial-mesenchymal transition, stemness, and metastasis via cytoplasmic translocation of active PTK6 and nuclear translocation of