- Authors: Daubeuf, S., Aucher, A., Bordier, C., Salles, A., Serre, L., Gaibelet, G., Faye, J. C., Favre, G., Joly, E., Hudrisier, D.
- Year: 2010
- Journal: PLoS One 5 e8716
- Applications: in vitro / DNA / jetPEI
- Cell type: HEK-293
Description: Human embryonic kidney Fibroblast
Known as: HEK293, 293
T and B cells capture antigens via membrane fragments of antigen presenting cells (APC) in a process termed trogocytosis. Whether (and how) a preferential transfer of some APC components occurs during trogocytosis is still largely unknown. We analyzed the transfer onto murine T and B cells of a large panel of fluorescent proteins with different intra-cellular localizations in the APC or various types of anchors in the plasma membrane (PM). Only the latter were transferred by trogocytosis, albeit with different efficiencies. Unexpectedly, proteins anchored to the PM's cytoplasmic face, or recruited to it via interaction with phosphinositides, were more efficiently transferred than those facing the outside of the cell. For proteins spanning the PM's whole width, transfer efficiency was found to vary quite substantially, with tetraspanins, CD4 and FcRgamma found among the most efficiently transferred proteins. We exploited our findings to set immunodiagnostic assays based on the capture of preferentially transferred components onto T or B cells. The preferential transfer documented here should prove useful in deciphering the cellular structures involved in trogocytosis.