Citation

  • Authors: Stefanov, A. N., Fox, J., Haston, C. K.
  • Year: 2013
  • Journal: PLoS Genet 9 e1003203
  • Applications: in vivo / DNA / in vivo-jetPEI

Method

Intravenous injection via tail vein was performed into B6 mice to target lungs. 40 µg of DNA were complexed with 6.5 µl of in vivo-jetPEI (N/P = 8) in a final volume of 400 µl. This led to a two times increase of transgene expression in the lungs compared to controls, without any increase in the tissue inflammatory response.

Abstract

Pulmonary fibrosis is a disease of significant morbidity, with no effective therapeutics and an as yet incompletely defined genetic basis. The chemotherapeutic agent bleomycin induces pulmonary fibrosis in susceptible C57BL/6J mice but not in mice of the C3H/HeJ strain, and this differential strain response has been used in prior studies to map bleomycin-induced pulmonary fibrosis susceptibility loci named Blmpf1 and Blmpf2. In this study we isolated the quantitative trait gene underlying Blmpf2 initially by histologically phenotyping the bleomycin-induced lung disease of sublines of congenic mice to reduce the linkage region to 13 genes. Of these genes, Trim16 was identified to have strain-dependent expression in the lung, which we determined was due to sequence variation in the promoter. Over-expression of Trim16 by plasmid injection increased pulmonary fibrosis, and bronchoalveolar lavage levels of both interleukin 12/23-p40 and neutrophils, in bleomycin treated B6.C3H-Blmpf2 subcongenic mice compared to subcongenic mice treated with bleomycin only, which follows the C57BL/6J versus C3H/HeJ strain difference in these traits. In summary we demonstrate that genetic variation in Trim16 leads to its strain-dependent expression, which alters susceptibility to bleomycin-induced pulmonary fibrosis in mice.

Pubmed