• Authors: Chen, X. Q., Liu, X., Wang, Q. X., Zhang, M. J., Guo, M., Liu, F., Jiang, W. F., Zhou, L.
  • Year: 2014
  • Journal: Exp Cell Res
  • Applications: in vitro / siRNA / INTERFERin
  • Cell type: Mouse atrial cardiac fibroblasts
    Description: Normal human dermal fibroblasts


10 nM


The exact mechanisms underlying inhibitory effects of pioglitazone (Pio) on Angiotensin II (AngII)-induced atrial fibrosis are complex and remain largely unknown. In the present study, we examined the effect of Pio on AngII-induced mice atrial fibrosis in vivo and atrial fibroblasts proliferation in vitro. In vivo study showed that AngII infusion induced atrial fibrosis and increased expressions of Toll/IL-1 receptor domain-containing adaptor inducing IFN-beta (TRIF) and tumor necrosis factor receptor associated factor 6 (TRAF6) in mice models. However, those effects could be attenuated by Pio (P<0.01). As for in vitro experiment, Pio suppressed AngII-induced atrial fibroblasts proliferation via nuclear factor-kappaB/transforming growth factor-beta1/TRIF/TRAF6 signaling pathway in primary cultured mice atrial fibroblasts (P<0.01). In conclusion, suppression of Pio on AngII-induced atrial fibrosis might be related to its inhibitory effects on above signaling pathway.