Citation

  • Authors: Peng, Y. P., Zhu, Y., Yin, L. D., Zhang, J. J., Wei, J. S., Liu, X., Liu, X. C., Gao, W. T., Jiang, K. R., Miao, Y.
  • Year: 2017
  • Journal: J Exp Clin Cancer Res 36 30
  • Applications: in vitro / in vivo / DNA, siRNA / jetPRIME, in vivo-jetPEI
  • Cell types:
    1. Name: CFPAC-1
      Description: Human pancreatic adenocarcinoma
    2. Name: PANC-1
      Description: Human pancreatic carcinoma cells

Method

Intratumoral injection with siRNA or negative control was performed once every 48 h during 4 weeks. siRNA and in vivo-jetPEI were separately added in 5% glucose solution, and mixed together. After a 15 min incubation time at room temperature, the siRNA/in vivo-jetPEI complexes were injected into animal.

Abstract

BACKGROUND: Overexpression of paternally expressed gene-10 (PEG10) is known to promote the progression of several carcinomas, however, its role in pancreatic cancer (PC) is unknown. We investigated the expression and function of PEG10 in PC. METHODS: PEG10 expression and correlation with PC progression was assessed in cancerous tissues and paired non-cancerous tissues. Further, the role of PEG10 in PC cell progression and the underlying mechanisms were studied by using small interfering RNA (Si-RNA). RESULTS: PEG10 expression was significantly higher in cancerous tissues and correlated with PC invasion of vessels and Ki-67 expression. Si-RNA mediated PEG10 knockdown resulted in inhibition of proliferation and G0/G1 cell cycle arrest, which was mediated by p21 and p27 upregulation. A decrease in PC cell invasion and migration, mediated by ERK/MMP7 pathway, was observed in PEG10 knockdown group. Further, findings of ChIP assay suggested that E2F-1 could directly enhance the expression of PEG10 through binding to PEG10 promoter. CONCLUSIONS: In conclusion, PEG10 was identified as a prognostic biomarker for PC and E2F-1 induced PEG10 could promote PC cell proliferation, invasion, and metastasis.

Pubmed