Citation

  • Authors: St-Denis, N., Gupta, G. D., Lin, Z. Y., Gonzalez-Badillo, B., Pelletier, L., Gingras, A. C.
  • Year: 2015
  • Journal: Mol Cell Proteomics 14 946-60
  • Applications: in vitro / DNA / jetPRIME
  • Cell types:
    1. Name: HEK-293T
      Description: Human embryonic kidney Fibroblast
      Known as: HEK293T, 293T
    2. Name: HeLa
      Description: Human cervix epitheloid carcinoma cells
    3. Name: U-2 OS
      Description: Human bone osteosarcoma
      Known as: U2OS

Abstract

The myotubularins are a family of phosphatases that dephosphorylate the phosphatidylinositols phosphatidylinositol-3-phosphate and phosphatidylinositol-3,5-phosphate. Several family members are mutated in disease, yet the biological functions of the majority of myotubularins remain unknown. To gain insight into the roles of the individual enzymes, we have used affinity purification coupled to mass spectrometry to identify protein-protein interactions for the myotubularins. The myotubularin interactome comprises 66 high confidence (false discovery rate

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