Citation
- Authors: Mishra N. et al.
- Year: 2022
- Journal: Data Brief 43 108415
- Applications: in vitro / DNA / FectoPRO
- Cell type: HEK-293F
Method
The variable domain D.N.A. was subcloned into pSCSTa-hIg1 and pSCST1-hk vectors. According to the manufacturer's instructions, vectors for the heavy and light chains were transfected into HEK293F cells using FectoPro (Polyplus Transfection).
Cell cultures were incubated for 4-5 days, the supernatant was collected, and protein-A affinity (∼2 mL packed beads per 600 mL culture) purifications were carried out. IgG proteins were eluted and buffer exchanged on PBS, pH 7.4.
Abstract
SARS-CoV-2 pandemic opens up the curiosity of understanding the coronavirus. This demand for the development of the regent, which can be used for academic and therapeutic applications. The present data provide the biochemical characterization of synthetically developed monoclonal antibodies for the SARS-CoV-2 proteins. The antibodies from phage-displayed antibody libraries were selected with the SARS-CoV-2 proteins immobilized in microwell plates. The clones which bind to the antigen in Fab-phage ELISA were selected, and a two-point competitive phage ELISA was performed. Antibodies binding kinetic of IgGs for SARS-CoV2 proteins further carried with B.L.I. Systematic analysis of binding with different control proteins and purified SARS-CoV-2 ensured the robustness of the antibodies.