Citation
- Authors: Hasabe A. et al.
- Year: 2020
- Journal: Methods Mol Biol 2210 195-204
- Applications: in vitro / Protein/Peptide/Antibody / PULSin
- Cell type: Mouse bone marrow-derived macrophages
Description: Primary mouse bone marrow macrophages
Known as: BMDM
Abstract
Microbial lipoproteins/lipopeptides are important virulence factors for periodontal diseases. The membrane lipoproteins from Mycoplasma salivarium or Tannerella forsythia can be easily extracted by exploiting a characteristic feature of Triton X-114: its aqueous nature at low temperatures (0-4 °C), which is absent at room temperature (25-37 °C). Transfection of these lipopeptides into macrophages was performed using the protein transfection reagent, PULSin.