• Authors: Munoz-Fontela, C., Collado, M., Rodriguez, E., Garcia, M. A., Alvarez-Barrientos, A., Arroyo, J., Nombela, C., Rivas, C.
  • Year: 2005
  • Journal: Exp Cell Res 311 96-105
  • Applications: in vitro / DNA / jetPEI
  • Cell types:
    1. Name: BCBL-1
    2. Name: COS-7
      Description: African green monkey kidney cells
      Known as: COS, COS7
    3. Name: HeLa
      Description: Human cervix epitheloid carcinoma cells
    4. Name: MCF7
      Description: Human breast adenocarcinoma cells
      Known as: MCF-7, MCF 7
    5. Name: MDA-MB-468
    6. Name: NIH/3T3
      Description: Murine embryonic fibroblasts
      Known as: NIH/3T3, 3T3


LANA2 is a latent protein detected in Kaposi's sarcoma-associated herpesvirus (KSHV)-infected B cells that inhibits p53-dependent transcriptional transactivation and apoptosis and PKR-dependent apoptosis, suggesting an important role in the transforming activity of the virus. It has been reported that LANA2 localizes into the nucleus of both KSHV-infected B cells and transiently transfected HeLa cells. In this study, we show that LANA2 is a nucleocytoplasmic shuttling protein that requires a Rev-type nuclear export signal located in the C-terminus to direct the protein to the cytoplasm, through an association with the export receptor CRM1. In addition, a functional protein kinase B (PKB)/Akt phosphorylation motif partially overlapping with the nuclear export signal was identified. Nuclear exclusion of LANA2 was negatively regulated by the phosphorylation of threonine 564 by Akt. The ability of LANA2 to shuttle between nucleus and cytoplasm has implications for the function of this viral protein.