Citation

  • Authors: Everett, K. L., Kraman, M., Wollerton, F. P. G., Zimarino, C., Kmiecik, K., Gaspar, M., Pechouckova, S., Allen, N. L., Doody, J. F., Tuna, M.
  • Year: 2018
  • Journal: Methods
  • Applications: in vitro / DNA / PEIpro
  • Cell type: HEK-293 6E
    Description: Human embryonic kidney Fibroblast cell line genetically modified with a truncated version of EBNA1 which grows in suspension and chemically defined serum-free medium.

Abstract

The immunoglobulin superfamily protein lymphocyte-activation gene 3 (LAG-3) participates in immune suppression and has been identified as a suitable target for cancer therapies. In order to generate bispecific antibodies targeting LAG-3, Fcabs (Fc-region with antigen binding) targeting human and murine LAG-3 were generated from phage libraries. These Fcabs bind to LAG-3, inhibiting its interaction with MHC class II, and induce IL-2 production in a T cell assay. Bispecific antibodies, known as mAb(2), were produced by replacing the Fc region of a monoclonal antibody with Fcab sequences in the CH3 domain. mAb(2) containing anti-LAG-3 Fcabs have mAb-like biophysical characteristics and retain LAG-3 binding and functional activity. mAb(2) can thus be generated using multiple Fabs to investigate bispecific parings and develop novel therapeutics.

Pubmed