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Citation

  • Authors: Kim, J. W., Oh, B. G., Kim, J., Kim, D. G., Nam, B. H., Kim, Y. O., Park, J. Y., Cheong, J., Kong, H. J.
  • Year: 2018
  • Journal: Dev Reprod 22 225-234
  • Applications: in vitro / DNA / jetPEI
  • Cell type: OFEC-17FEN
    Description: Embryonic cell line derived from olive flounder Paralichthys olivaceu.

Abstract

A new embryonic cell line (OFEC-17FEN) derived from olive flounder Paralichthys olivaceus was developed. OFEC-17FEN cells were subcultured for <30 passages over ~200 days. OFEC-17FEN cells had a doubling time of 114.34 h and modal diploid chromosome number was 48. The pluripotency genes POU5f1 and NANOG were expressed in OFEC-17FEN cells. However, the lack of several pluripotency-related genes expression indicates that OFEC-17FEN cells are not stem cells. OFEC-17FEN cells transfected with plasmid pEGFP-c1 exhibited a strong green fluorescent signal at 48 h after transfection. Accordingly, OFEC-17FEN cells may be useful for both basic research and biotechnological application.

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