- Authors: Dandekar, P., Jain, R., Keil, M., Loretz, B., Muijs, L., Schneider, M., Auerbach, D., Jung, G., Lehr, C. M., Wenz, G.
- Year: 2012
- Journal: J Control Release
- Applications: in vitro / DNA / jetPRIME
- Cell type: A549
Description: Human lung carcinoma cells, type II pneumocytes
Known as: A-549
Cationic polyrotaxanes, obtained by temperature activated threading of cationic cyclodextrin derivatives onto water-soluble cationic polymers (ionenes), form metastable nanometric polyplexes with pDNA and combinations of siRNA with pDNA. Because of their low toxicity, the polyrotaxane polyplexes constitute a very interesting system for the transfection of polynucleotides into mammalian cells. The complexation of Cy3-labeled siRNA within the polyplexes was demonstrated by fluorescence correlation spectroscopy. The uptake of the polyplexes (red) was imaged by confocal fluorescence microscopy using the A549 cell line as a model (blue: nuclei, green: membranes). The results prove the potential of polyrotaxanes for further investigations involving knocking down genes of therapeutic interest.