Citation

  • Authors: Lee, J. J., Lee, J. H., Yim, N. H., Han, J. H., Ma, J. Y.
  • Year: 2017
  • Journal: Sci Rep 7 8207
  • Applications: in vivo / siRNA / in vivo-jetPEI

Method

p27 siRNA and in vivo-jetPEI were combined into a complex. siRNA solution in 5 % glucose (150 µg/mL) was mixed with in vivo-jetPEI and then allowed to stand for at least 30 min at room temperature. This complex was injected intravenously four times over 14 days.

Abstract

In clinical pathology, stent interposition is used to treat vascular disease but can lead to restenosis. Drug-eluting stents (DES) are most commonly used to suppress restenosis but can also have side effects. Therefore, we investigated the anti-proliferative effect and its possible target in vitro and in vivo. We found that Alpinia officinarum Hance (AO) extract efficiently inhibited VSMC proliferation by arresting the transition from the G0/G1 to the S phase via the up-regulation of p27(KIP1) expression. Galangin (GA) was determined to be a significant component of this extract, with the same anti-proliferative activity as the raw extract. Immunoblotting and immunofluorescence staining showed that both the AO extract and GA targeted the up-regulation of p27(KIP1) expression. Therefore, we next examined the effect of these compounds in a cuff-injured neointimal hyperplasia model in vivo. In this animal model, both the AO extract and GA completely suppressed the neointima formation, and this inhibitory effect was also demonstrated to target the up-regulation of p27(KIP1), including the suppression of proliferating cell nuclear antigen expression. Our findings indicate that AO extract and GA have a potent anti-proliferative activity, targeting the up-regulation of p27 expression. Thus, GA may represent an alternative medicine for use in DES.

Pubmed