The use of the CRISPR/Cas9 system in mammalian cells has recently emerged as a very convenient way to modify the cell genome at a specific locus. It involves transient transfection into mammalian cells of either (a) one or several plasmids coding for Cas9, the specific gRNA and eventually the sequence to be inserted, or (b) a mix of one or two plasmids and an RNA molecule (the gRNA).
jetPRIME® is a new powerful and versatile DNA and siRNA transfection reagent for day-to-day experiments. jetPRIME® ensures high DNA transfection efficiency and excellent gene silencing in a variety of adherent cells. jetPRIME® is ideal for DNA/siRNA co-transfection, hence it is perfectly well-suted for gene editing applications in which the CRISPR/Cas9 system need to be transiently expressed, following transfection. In addition, jetPRIME® is very gentle on cells since it requires low amounts of reagent and nucleic acid during transfection, making it the reagent of choice for sensitive cells.
For more information, read the jetPRIME – CRISPR Application Note.