CRISPR-Cas9: overview, challenges and latest technologies
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Check out this new webinar made by our experts in transfection on CRISPR-Cas9 genome editing system. When using CRISPR/Cas9 system, transfection is a key and often limiting step to ensure successful genome editing.genom
There are three main systems to transfect guide RNA and express Cas9 protein which are DNA, RNA or RNP (gRNA + Cas9 protein) approaches. Each system has their own pros and cons in terms of easiness of use, genome editing efficiency and off-targeting effects.