Meet our team booth #43 during the BioProcess International European Summit, in Amsterdam (Netherlands), April 23th – 25th, 2018.
Development and Optimization of an Advanced Transient Expression System in CHO cells
The growing need in the biopharmaceutical market for various recombinant proteins and antibodies produced in a short time requires an efficient and flexible system. Transient expression is a commonly used process to face this demand but is unfortunately limited by transfection efficiency and inherent productivity, especially with CHO cells. To overcome this issue, we developed an advanced transient expression system consisting in the synergistic association of a novel CHO chemically defined medium and a powerful transfection reagent. First, we show that this innovative medium allows easy cultivation of various strains of CHO cells without the need of an extensive sequential adaptation. In a second time, through the protocol optimization of a technologically advanced transfection solution, we demonstrate a major increase in our recombinant proteins yields.
Our solutions for both protein and virus production
FectoPRO® Transfection kit is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount (< 1 µg/ml of cell culture).
PEIpro® is a DNA transfection reagent for production of viruses, recombinant proteins and antibodies either in adherent cells in presence of serum or in suspension HEK-293 cells grown in low serum or serum-free media.
PEIpro®-HQ is a highly qualified grade of PEIpro® reagent, especially developed for the production of clinical batches of recombinant proteins, antibodies or viral vectors. The PEIpro®-HQ grade is supplied with appropriate Quality Controls and documentation allowing its use as a qualified raw material in GMP processes for the manufacturing of clinical batches of therapeutic products.
The convention will take place in RAI Amsterdam:
Europaplein 2-22, 1078 GZ Amsterdam, Netherlands
Meet our team to discuss how to improve your process of Bioproduction. We will be happy to answer all your questions regarding transfection and to present relevant products adapted to protein or virus production.
Scientific and Technical Support Specialist
Senior Scientist in Chemistry