Protein production

Transient gene expression (TGE) is commonly used for medium scale production of recombinant proteins and antibodies in CHO and HEK-293 cells. This approach allows generation of sufficient protein amounts avoiding a major investment in production of stable cell lines prior to “proof of concept” studies or tools validation. Indeed, the speed and flexibility of TGE has enabled this technique to be widely adopted in bioproduction for early discovery, research applications and process developments. Polyplus-transfection has developed a range of products specifically designed for protein production in CHO and HEK-293 cells grown in suspension in synthetic serum-free media.