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Home > Gene expression

Application : Gene expression
Gene expression

Process of gene expression entails plasmid DNA or mRNA delivery to specific cells and is a technique routinely utilized in labs to study a gene of interest or a signaling pathway. Gene expression usually allows transient protein production, but in some cases stable integration into a genome and continuous protein production over time is required. In addition, different cell types show specific requirements for successful gene expression and thus each represent unique challenges. Polyplus-transfection offers solutions for each of these processes as well as reagents for a wide variety of cells including hard to transfect cells.

Best-in-class DNA transfection reagent
jetOPTIMUS®
mRNA transfection reagent for hard to transfect cells
jetMESSENGER®
DNA transfection, HTS application
jetPEI®
For primary hepatocytes
jetPEI®-Hepatocyte
For endothelial cells
jetPEI®-HUVEC
For primary macrophages, glial & dendritic cells
jetPEI®-Macrophage
Versatile DNA/siRNA transfection reagent
jetPRIME®
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67400 Illkirch
FRANCE

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NY 10020 USA

Ph: +33 (0)3 90 40 61 80
Fax: +33 (0)3 90 40 61 81

Applications
  • Gene expression
  • Genome editing
  • RNA interference
  • Protein production
  • Virus Production
  • in vivo delivery
  • Therapeutics
  • Molecular Biology & Diagnostics
  • Protein delivery
  • Custom Services
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AFAQ - ISO 9001 2019 - Copyright Polyplus Transfection®
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Gene expression
Gene expression
Plasmid DNA & mRNA transfection, Genome editing, Cell-specific DNA reagents
Genome editing
Genome editing
Delivery of gRNA and Cas9 for all systems
RNA interference
RNA interference
Transfection of siRNA, miRNA or other oligonucleotides
Protein production
Protein production
Large scale TGE, high protein yields
Virus Production
Virus Production
Large scale virus production, clinical batches production, AAV, lentivirus
in vivo delivery
in vivo delivery
in vivo delivery of nucleic acids in various tissues of different animal models
Therapeutics
Therapeutics
Reagents for preclinical and clinical trials in human, Therapeutics oligonucleotides
Molecular Biology & Diagnostics
Molecular Biology & Diagnostics
PCR, primer, probe
Protein delivery
Protein delivery
Intracellular delivery of proteins, peptides and blocking antibodies to live cells
Custom Services
Custom Services
Custom-made products, personnalized transfection services, custom quality controls
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ProductsDelivered MoleculesCell typesRecommended use
DNAsiRNA/miRNAmRNAProteins Adherent SuspensionSpecific
In vitro
jetOPTIMUS® Hard to transfect cellsResearch purpose
jetPRIME® Research purpose
INTERFERin® RNA interference
jetMESSENGER® Hard to transfect cellsResearch purpose
jetCRISPR™ CRISPR/Cas9 experiment
FectoPRO® Suspension CHO & HEK-293Protein production
FectoCHO™ Expression System CHO cellsProtein production
PEIpro® Product Range HEK-293Virus Production
PEIpro® HEK-293Large-scale Virus Production
PEIpro®-HQ HEK-293Pre-clinical and early phase clinical virus production
PEIpro®-GMP HEK-293Clinical trials and commercialization
jetPEI® High Throughput Screening Research purpose
jetPEI®-Macrophage Primary macrophages, glial & dendritic cellsCell specific
jetPEI®-Hepatocyte Primary HepatocytesCell specific
jetPEI®-HUVEC Endothelial cellsCell specific
PULSin Proteofection
ProductsPlasmid DNAOligo-nucleotidesmRNAProteinsAdherentSuspensionSpecificRecommended use
In vivo
in vivo-jetPEI®in vivo delivery to all organs
Preclinical and Cell Therapy reagentsTherapeutic in vivo delivery to all organs
cGMP grade in vivo-jetPEI®Therapeutic in vivo delivery to all organs
in vivo-jetPEI®-Manin vivo delivery delivery to mannose-receptor expressing tissue
in vivo-jetPEI®-Galin vivo delivery to liver
jetSI 10 mMsiRNA delivery to brain
ProductsPlasmid DNAOligo-nucleotidesmRNAProteinsAdherentSuspensionSpecificRecommended use
Other
SpCas9 NucleaseCRISPR/Cas9 experiment
Zip Nucleic acid® (ZNA®)Molecular Biology & Diagnostics
STICKY SIRNATherapeutic siRNA
OLIGOPLUS and SIRNAPLUS®Therapeutic cationic Oligonucleotides
Personalized transfection servicesTransfection optimization
Custom-made productsin vitro transfection, therapeutics
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For cell lines and references, please search the Polyplus-transfection Database.

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