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Home > Gene expression

Application : Gene expression
Gene expression

Process of gene expression entails plasmid DNA or mRNA delivery to specific cells and is a technique routinely utilized in labs to study a gene of interest or a signaling pathway. Gene expression usually allows transient protein production, but in some cases stable integration into a genome and continuous protein production over time is required. In addition, different cell types show specific requirements for successful gene expression and thus each represent unique challenges. Polyplus-transfection offers solutions for each of these processes as well as reagents for a wide variety of cells including hard to transfect cells.

Best-in-class DNA transfection reagent
jetOPTIMUS®
mRNA transfection reagent for hard-to-transfect cells
jetMESSENGER®
DNA transfection, HTS application
jetPEI®
For primary hepatocytes
jetPEI®-Hepatocyte
For endothelial cells
jetPEI®-HUVEC
For primary macrophages, glial & dendritic cells
jetPEI®-Macrophage
Versatile DNA/siRNA transfection reagent
jetPRIME®
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Applications
  • Gene expression
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  • RNA interference
  • Protein production
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AFAQ - ISO 9001 2019 - Copyright Polyplus Transfection®
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Gene expression
Gene expression
Plasmid DNA & mRNA transfection, Genome editing, Cell-specific DNA reagents
Genome editing
Genome editing
Delivery of gRNA and Cas9 for all systems
RNA interference
RNA interference
Transfection of siRNA, miRNA or other oligonucleotides
Protein production
Protein production
Large scale TGE, high protein yields
Virus Production
Virus Production
Large scale virus production, clinical batches production, AAV, lentivirus
in vivo delivery
in vivo delivery
in vivo delivery of nucleic acids in various tissues of different animal models
Therapeutics
Therapeutics
Reagents for preclinical and clinical trials in human, Therapeutics oligonucleotides
Molecular Biology & Diagnostics
Molecular Biology & Diagnostics
PCR, primer, probe
Protein delivery
Protein delivery
Intracellular delivery of proteins, peptides and blocking antibodies to live cells
Custom Services
Custom Services
Custom-made products, personnalized transfection services, custom quality controls
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Products Delivered Molecules Cell types Recommended use
DNA siRNA/miRNA mRNA Proteins  Adherent  Suspension Specific
In vitro
jetOPTIMUS® Hard to transfect cells Research purpose
jetPRIME® Research purpose
INTERFERin® RNA interference
jetMESSENGER® Hard-to-transfect cells Research purpose
jetCRISPR® CRISPR/Cas9 experiment
FectoPRO® Suspension CHO & HEK-293 Protein production
FectoCHO® Expression System CHO cells Protein production
PEIpro® Product Range HEK-293 Virus Production
PEIpro® HEK-293 Large-scale Virus Production
PEIpro®-HQ HEK-293 Pre-clinical and early phase clinical virus production
PEIpro®-GMP HEK-293 Clinical trials and commercialization virus production
jetPEI® High Throughput Screening Research purpose
jetPEI®-Macrophage Primary macrophages, glial & dendritic cells Cell specific
jetPEI®-Hepatocyte Primary Hepatocytes Cell specific
jetPEI®-HUVEC Endothelial cells Cell specific
PULSin Proteofection
Products Plasmid DNA Oligo-nucleotides mRNA Proteins Adherent Suspension Specific Recommended use
In vivo
in vivo-jetRNA® in vivo mRNA transfection
in vivo-jetPEI® Product Range in vivo transfection
in vivo-jetPEI® in vivo transfection to all organs
in vivo-jetPEI®-PC Injectable in vivo transfection reagent
GMP in vivo-jetPEI® Therapeutic in vivo transfection to all organs
in vivo-jetPEI®-Man in vivo delivery to mannose-receptor expressing tissue
in vivo-jetPEI®-Gal in vivo delivery to liver
jetSI 10 mM siRNA transfection to brain
Products Plasmid DNA Oligo-nucleotides mRNA Proteins Adherent Suspension Specific Recommended use
Other
SpCas9 Nuclease CRISPR/Cas9 experiment
Zip Nucleic acid® (ZNA®) Molecular Biology & Diagnostics
STICKY SIRNA Therapeutic siRNA
OLIGOPLUS and SIRNAPLUS® Therapeutic cationic Oligonucleotides
Personalized transfection services Transfection optimization
Custom-made products in vitro transfection, therapeutics
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For cell lines and references, please search the Polyplus-transfection Database.

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