Choose among a broad range of powerful transfection reagents specifically developed to enable you to reach the desired level of protein expression in a wide range of adherent and suspension mammalian cell types, primary and cell lines. Transfection reagents are ready-to-use with a pre-optimized protocol to ensure high transfection efficiency while preserving cell viability and morphology.

Applications 5

jetOPTIMUS packaging 2020
jetOPTIMUS®
in vitro
Best-in-class DNA transfection reagent
Plasmid DNA
jetPRIME packaging 2020
jetPRIME®
in vitro
Versatile DNA/siRNA transfection reagent
Plasmid DNA,Oligonucleotides (siRNA, miRNA and Other)
INTERFERin packaging 2020
INTERFERin®
in vitro
siRNA, miRNA and other oligonucleotides
Oligonucleotides (siRNA, miRNA and Other)
jetMESSENGER packaging 2020
jetMESSENGER®
in vitro
mRNA transfection reagent for hard-to-transfect cells
mRNA
jetPEI packaging 2020
jetPEI®
in vitro
DNA transfection, HTS application
Plasmid DNA
PULSin packaging 2020
PULSin®
in vitro
Protein, antibody and peptide delivery reagent
Protein, peptide & antibodies

Gene and Cell Therapy rely on the use of modified viruses as vectors to transfer genes into cells that they are able to infect.  Modified viruses used as vectors are derived from adeno-associated viruses (AAV), lentiviruses, retroviruses and adenoviruses. These viral vectors first need to be produced in mammalian cells. Production of viral vectors is dependent on efficient transfection of one to several plasmid DNA containing the necessary viral genome to produce recombinant viral particles.

Applications 2

FectoVIR-AAV GMP
FectoVIR®-AAV GMP
in vitro
GMP grade AAV manufacturing
Plasmid DNA
FectoVIR-AAV - bottle packaging 2020
FectoVIR®-AAV
in vitro
Recombinant AAV virus production in suspension cells
Plasmid DNA
PEIpro-GMP packaging 2020
PEIpro®-GMP
in vitro
The highest quality grade for therapeutic virus production
Plasmid DNA
PEIpro®
in vitro
Large-scale virus Production
Plasmid DNA
PEIpro®-HQ
in vitro
Pre-clinical and early phase clinical virus production
Plasmid DNA
Polyplus_hplc
Residual PEIpro® test
in vitro
GMP-compliant
-

Protein production consists in overexpressing recombinant proteins (eg. antibodies) and peptides mainly in mammalian cell lines HEK-293, CHO and derivatives. Transfection of plasmid DNA encoding for the protein of interest in a given cell line can be used for both transient and stable protein production. Transient protein expression allows generation of high protein amounts in a short amount of time and can offer a reliable alternative to production of stable cell lines for proof of concept studies and tools validation. Polyplus-transfection has developed a range of products specifically designed for protein production in CHO and HEK-293 cells grown in suspension in synthetic serum-free media.

FectoPRO packaging 2020
FectoPRO®
in vitro
High yields in suspension CHO & HEK-293
Plasmid DNA
FectoCHO packaging 2020
FectoCHO® Expression System
in vitro
Enhanced transient protein production in CHO cells
Plasmid DNA

Non-viral in vivo delivery reagents are the most powerful alternative to viral vectors for nucleic acid delivery. They offer substantial advantages in terms of reliability, safety and costs for nucleic-acid based therapies. Different quality grades of in vivo transfection reagents are available for proof concept studies, preclinical studies and clinical trials.

Applications 2

LipidBrick® IM21.7c
in vivo
Cationic lipids for LNP formulation
mRNA
in vivo-jetRNA®+
in vivo
in vivo mRNA delivery using liposome-based reagent
mRNA
in vivo-jetPEI®
in vivo
in vivo DNA, si/miRNA transfection
Plasmid DNA,Oligonucleotides (siRNA, miRNA and Other)
in vivo-jetPEI-PC packaging 2020
in vivo-jetPEI®-PC
in vivo
Injectable in vivo transfection reagent
Plasmid DNA,Oligonucleotides (siRNA, miRNA and Other)
GMP in vivo-jetPEI packaging 2020
GMP in vivo-jetPEI®
in vivo
Non-viral delivery reagent for Human Gene Therapy
Plasmid DNA,Oligonucleotides (siRNA, miRNA and Other)

Polyplus Transfection – The transfection experts

Polyplus is a leading upstream solutions provider for advanced biologic and cell and gene therapy production from research to commercial scale. An innovator in nucleic acid delivery, the legacy portfolio features process-centric transfection reagents, kits, and support services. Custom plasmid vector design was integrated into the offer in 2022 as a first measure to expanding the products and services portfolio to help customers (or the industry) optimize process economics while meeting strict scientific and regulatory standards. Headquartered in Europe, the Polyplus team continues to grow globally with operations in the United States and Asia.

Search in our Transfection Database

Search in the publication database with Polyplus transfection reagents or for transfection conditions
(over 4000 publications, 1000 cell lines and primary cells available).

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