Polyplus-transfection news
18 March 2020
Communication regarding the COVID-19
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Choose among a broad range of powerful transfection reagents specifically developed to enable you to reach the desired level of protein expression in a wide range of adherent and suspension mammalian cell types, primary and cell lines. Transfection reagents are ready-to-use with a pre-optimized protocol to ensure high transfection efficiency while preserving cell viability and morphology.
Applications 6
Gene and Cell Therapy rely on the use of modified viruses as vectors to transfer genes into cells that they are able to infect. Modified viruses used as vectors are derived from adeno-associated viruses (AAV), lentiviruses, retroviruses and adenoviruses. These viral vectors first need to be produced in mammalian cells. Production of viral vectors is dependent on efficient transfection of one to several plasmid DNA containing the necessary viral genome to produce recombinant viral particles.
Protein production consists in overexpressing recombinant proteins (eg. antibodies) and peptides mainly in mammalian cell lines HEK-293, CHO and derivatives. Transfection of plasmid DNA encoding for the protein of interest in a given cell line can be used for both transient and stable protein production. Transient protein expression allows generation of high protein amounts in a short amount of time and can offer a reliable alternative to production of stable cell lines for proof of concept studies and tools validation. Polyplus-transfection has developed a range of products specifically designed for protein production in CHO and HEK-293 cells grown in suspension in synthetic serum-free media.
Non-viral in vivo delivery reagents are the most powerful alternative to viral vectors for nucleic acid delivery. They offer substantial advantages in terms of reliability, safety and costs for nucleic-acid based therapies. Different quality grades of in vivo transfection reagents are available for proof concept studies, preclinical studies and clinical trials.
Applications 3
Polyplus-transfection® SA is the leading biotechnology company that supports Gene and Cell therapy, biologics manufacturing and life science research with innovative nucleic acid transfection solutions. Polyplus-transfection’s strengths are 20 years of experience in manufacturing transfection solutions with tailored scientific and regulatory support to accelerate research.
Based on the Science Park close to Strasbourg (France), Polyplus-transfection® offers an extensive and growing range of transfection reagents available worldwide.
Search in our Transfection Database
Search in the publication database with Polyplus transfection reagents or for transfection conditions
(over 4000 publications, 1000 cell lines and primary cells available).
Newsroom
Blog
Case study
18 November 2019
Improving expression and purification of mAb using FectoCHO® Expression System and MabXpure™.
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Podcast
15 October 2019
Identifying and mitigating risks in the viral vector supply chain
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Polyplus-transfection news
8 October 2019
[NEW] Annual Brochure 2019 – Transfection reagents for protein & viral vector production
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