By simple and rapid transient transfection method, FectoFly™ provides high transfection efficiency in insect cells and robust protein production over several days (Fig. 1).

Fig 1. Protein production in S2 cells over 5 days using FectoFly transfection reagent. A suspension culture of 106 cells/ml was transfected using 2 µg pCMV-EGFPLuc and 6.4 µl of FectoFly per ml of culture in Insect-XPRESS™ medium (Lonza).

FectoFly™ allows successful transfection of adherent and suspension insect cell cultures both in the latest generation of syntheticserum-free media (Fig.1, 3 – Sf21 and Sf9) and in the presence of serum (Fig.3 – S2). Transfection efficiency ranges routinely from 30% to 50% and cell viability is above 80%.

		Fig 3. GFP expression in Sf21, Sf9 cells grown in suspension, 72 h after transfection of  pCMV-EGFP-Luc plasmid using FectoFly™.

FectoFly™ was compared to other commercially available reagents dedicated to the transfection of insect cells (Fig. 4). As shown for Sf9 cells, the highest protein expression levels were obtained with FectoFly™.

Fig 4. Comparison of FectoFly™ with other insect cell-specific transfection reagents. Sf9 adherent cells were transfected in 6-well plates with pCMV-EGFPLuc plasmid using insect cell transfection reagents according to the supplier’s recommendations.