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Insect Cells: FectoFly®

  • High protein expression and baculovirus production
  • Global transfection solution for insect cells
  • High transfection efficiency in adherent and suspension insect cells
  • Guaranteed free of material from animal origin

FectoFly® is a DNA transfection reagent dedicated to insect cells for high level production of baculovirus and recombinant proteins.

FectoFly® is also well-suited for high level transgene expression in insect cells.

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Ordering information
Product Catalog Number Amount of reagent Amount of 150 mM NaCl solution
FectoFly® 112-01N 0.1 mL 5 mL
112-10N 1 mL 50 mL

1 ml of FectoFly® is sufficient to perform 100 to 200 transfections in 6-well plates.

For bulk sizes, Please contact us.

 
Robust protein production
By simple and rapid transient transfection method, FectoFly® provides high transfection efficiency in insect cells and robust protein production over several days (Fig. 1).

FectoFly Fig1 Fig 1. Protein production in S2 cells over 5 days using FectoFly® transfection reagent. A suspension culture of 106 cells/ml was transfected using 2 µg pCMV-EGFPLuc and 6.4 µl of FectoFly® per ml of culture in Insect-XPRESS™ medium (Lonza).

 
Adapted to a range of insect cells
FectoFly® provides high transfection efficiency in most commonly used insect cells (Fig. 2) allowing efficient protein production.

Figure 2. Comparison of FectoFly® in various adherent cell lines. Sf9, Sf21, S2 or Tn5 adherent cells were transfected in 24-well plates pCMV-EGFP-Luc plasmid. Luciferase assays were performed 72 h after transfection.

FectoFly Fig2vB Fig. 2. Comparison of FectoFly® in various adherent cell lines. Sf9, Sf21, S2 or Tn5 adherent cells were transfected in 24-well plates pCMV-EGFP-Luc plasmid. Luciferase assays were performed 72 h after transfection.

 
Suitable for both adherent and suspension cells

FectoFly® allows successful transfection of adherent and suspension insect cell cultures both in the latest generation of syntheticserum-free media (Fig.1, 3 – Sf21 and Sf9) and in the presence of serum (Fig.3 – S2). Transfection efficiency ranges routinely from 30% to 50% and cell viability is above 80%.

FectoFly Sf21 FectoFly Sf9 Fig 3. GFP expression in Sf21, Sf9 cells grown in suspension, 72 h after transfection of  pCMV-EGFP-Luc plasmid using FectoFly®.
FectoFly S2

 
Superior protein production levels

FectoFly® was compared to other commercially available reagents dedicated to the transfection of insect cells (Fig. 4). As shown for Sf9 cells, the highest protein expression levels were obtained with FectoFly®.

FectoFly Fig4 Fig 4. Comparison of FectoFly® with other insect cell-specific transfection reagents. Sf9 adherent cells were transfected in 6-well plates with pCMV-EGFPLuc plasmid using insect cell transfection reagents according to the supplier’s recommendations.

 
Extremely simple protocol

Transfection of insect cells with FectoFly® is straightforward.
o Fast : 3 steps protocol
o Simple: 1 µl of FectoFly® per µg of DNA, 1:1 ratio
o Versatile: efficient in serum-free synthetic media and in the presence of serum

Figure 5. FectoFly® standard protocol

 FectoFly Protocol