FectoFly™ « Polyplus Transfection

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Bioproduction in Insect Cells: FectoFly™

High protein expression and baculovirus production
Global transfection solution for insect cells
High transfection efficiency in adherent and suspension insect cells
Guaranteed free of material from animal origin

FectoFly™ is a DNA transfection reagent dedicated to insect cells for high level production of baculovirus and recombinant proteins.

FectoFly™ is also well-suited for high level transgene expression in insect cells.

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Ordering information

Product	Catalog Number	Amount of reagent	Amount of 150 mM NaCl solution
	112-01N	0.1 mL	5 mL
FectoFly™	112-10N	1 mL	50 mL
	112-40N	4 x 1 mL	4 x 50 mL

1 ml of FectoFly™ is sufficient to perform 100 to 200 transfections in 6-well plates.

For bulk sizes, Please contact us.

Robust protein production

By simple and rapid transient transfection method, FectoFly™ provides high transfection efficiency in insect cells and robust protein production over several days (Fig. 1).

Fig 1. Protein production in S2 cells over 5 days using FectoFly transfection reagent. A suspension culture of 106 cells/ml was transfected using 2 µg pCMV-EGFPLuc and 6.4 µl of FectoFly per ml of culture in Insect-XPRESS™ medium (Lonza).

Adapted to a range of insect cells

FectoFly™ provides high transfection efficiency in most commonly used insect cells (Fig. 2) allowing efficient protein production.

Figure 2. Comparison of FectoFly™ in various adherent cell lines. Sf9, Sf21, S2 or Tn5 adherent cells were transfected in 24-well plates pCMV-EGFP-Luc plasmid. Luciferase assays were performed 72 h after transfection.

Suitable for both adherent and suspension cells

FectoFly™ allows successful transfection of adherent and suspension insect cell cultures both in the latest generation of syntheticserum-free media (Fig.1, 3 – Sf21 and Sf9) and in the presence of serum (Fig.3 – S2). Transfection efficiency ranges routinely from 30% to 50% and cell viability is above 80%.

		Fig 3. GFP expression in Sf21, Sf9 cells grown in suspension, 72 h after transfection of pCMV-EGFP-Luc plasmid using FectoFly™.

Superior protein production levels

FectoFly™ was compared to other commercially available reagents dedicated to the transfection of insect cells (Fig. 4). As shown for Sf9 cells, the highest protein expression levels were obtained with FectoFly™.

Fig 4. Comparison of FectoFly™ with other insect cell-specific transfection reagents. Sf9 adherent cells were transfected in 6-well plates with pCMV-EGFPLuc plasmid using insect cell transfection reagents according to the supplier’s recommendations.

Extremely simple protocol

Transfection of insect cells with FectoFly™ is straightforward.
o Fast : 3 steps protocol
o Simple: 1 µl of FectoFly™ per µg of DNA, 1:1 ratio
o Versatile: efficient in serum-free synthetic media and in the presence of serum

Figure 5. FectoFly™ standard protocol.