jetSI™ 10 mM

siRNA delivery to the brain

  • Unique reagent for siRNA delivery to the brain
  • Successful siRNA delivery to the brain
  • Low toxicity induced

Specifications

Reagent

jetSI™ 10 mM

Molecule delivered

siRNA

Applications

in vivo siRNA delivery

Targeted organs

Brain

Injection route

Intracranial injection
Stereotaxical injection
Intraventricular injection

Number of transfections

500 µl of jetSI™ 10 mM delivery reagent is sufficient to deliver up to 2.5 mg of siRNA to mouse brain (c.a. 100 ng of siRNA per injection).

Storage

4 °C, for at least 6 months

Summary

in vivo-jetPEI® is broadly used as an in vivo delivery reagent. However, for siRNA delivery to the brain, Polyplus-transfection® has developed jetSI™ 10 mM.

The choice of the most effective siRNA carrier may depend on the in vivo context. Indeed, while in vivo-jetPEI® is superior to cationic liposomes for plasmid DNA delivery in the mouse brain, jetSI™ 10 mM was found to be the carrier of choice for siRNA delivery to this organ (Froidevaux et al. (2006), EMBO Rep 7:1035; Guissouma et al. (2006), Neurosci Lett 406: 240; Kumar et al. (2006), PLOS Med. 3: 0505; Hassani et al. (2005), J Gene Med 7:198).

When using plasmid based approaches (shRNA) in the brain, we recommend in vivo-jetPEI®.

Ordering information

Catalog NumberAmount of reagent
403-050.5 ml

in vivo functional studies

jetSI™ 10 mM is perfectly suited to study gene function in the brain in vivo and provides a useful method for the validation of in vitro functional studies into animals.

Here is a selection of relevant references using jetSI™ 10 mM, more are available in our Polyplus-transfection Database.

Cheret, C., Gervais, A., Lelli, A., Colin, C., Amar, L., Ravassard, P., Mallet, J., Cumano, A., Krause, K. H., Mallat, M. (2008). Neurotoxic activation of microglia is promoted by a nox1-dependent NADPH oxidase., J Neurosci 28, 12039.

Griggs, E. M., Young, E. J., Rumbaugh, G., Miller, C. A. (2013). MicroRNA-182 regulates amygdala-dependent memory formation., J Neurosci 33, 1734.

Karatas, H., Erdener, S. E., Gursoy-Ozdemir, Y., Lule, S., Eren-Kocak, E., Sen, Z. D., Dalkara, T. (2013). Spreading depression triggers headache by activating neuronal Panx1 channels., Science 339, 1092.

Tai, D. J., Hsu, W. L., Liu, Y. C., Ma, Y. L., Lee, E. H. (2011). Novel role and mechanism of protein inhibitor of activated STAT1 in spatial learning., Embo J 30, 205-2.

Young, E.J., Blouin, A.M., Briggs, S.B., Sillivan, S.E., Lin, L., Cameron, M.D., Rumbaugh, G., Miller, C.A. (2015). Nonmuscle myosin IIB as a therapeutic target for the prevention of relapse to methamphetamine use., Mol Psychiatry. doi: 10.1038/mp.2015.103.

« I finished the experiments and I think in vivo-jetPEI works great. I am very pleased with the results. Actually some of the work we have done with in vivo-jetPEI® as well as jetSI™ 10 mM has been published in Science. I would like to tell you that I am also very pleased with the technical assistance. Many thanks! »

E.E.K., Hacettepe University, Turkey

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