jetMESSENGER™

mRNA transfection reagent for hard to transfect cells

  • Unmatched transfection efficiency
  • High efficiency on a wide variety of difficult to transfect cells
  • Outperform DNA transfection by switching to mRNA
  • Extremely gentle on cells
  • No risk of genome integration
  • Perfectly suited for CRISPR/Cas9 gene editing, iPS generation, stem cell differentiation and immunotherapy assays

Specifications

Reagent

jetMESSENGER™

Molecule delivered

mRNA

Applications

Gene expression
Genome Editing
Stem cell differentiation
Reprogramming
Immunotherapy

Cell types

Hard to transfect cells
Adherent and suspension cells
Primary cells (Neurons, Stem cells, Immune cells, fibroblasts, …)
Post-mitotic cells

Number of transfections

1.5 ml of jetMESSENGER™ transfection reagent is sufficient to perform up to 1500 transfections in 24-well plates or 375 transfections in 6-well plates

Storage

4 °C, stable for at least one year when stored appropriately

Provided with

mRNA buffer

Summary

Take your gene expression to the next level by switching to mRNA!

Primary cells, neurons, suspension cells and various cancer cell lines are especially challenging to transfect. jetMESSENGER™ gives high transfection efficiency in all of these usually difficult to transfect cells, by allowing highly efficient mRNA transfection.

mRNA transfection is as easy as DNA transfection, with the advantage that mRNA does not need to reach the cell nucleus for expression nor require cell division for efficient gene expression. Hence, cells that are slow dividing or that have developed specific mechanisms to protect their genome can finally be used for gene expression.

Unravel the full potential of your cells by switching to mRNA transfection.

Polyplus-transfection - Trends in Transfection A video presenting mRNA transfection for hard to transfect cells is available

Protocols and Files

Ordering information

Catalog NumberAmount of reagentAmount of buffer
150-010.1 ml10 ml
150-070.75 ml60 ml
150-151.5 ml2 x 60 ml

1.5 ml is sufficient to perform ca.375 transfections in 6-well plates. Bulk quantities are available upon request. Please contact us.

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Outperforms DNA transfection every time

jetMESSENGER™ is a highly efficient and gentle mRNA transfection reagent. mRNA transfection presents many advantages versus DNA transfection:

  • no risk of genome integration, hence no genome modification of the transfected cell
  • no promoter regulation issue
  • no need to reach the nucleus for efficient expression
  • more gentle process

jetMESSENGER™ has been specifically designed to offer outstanding transfection efficiency in cells that are usually hard to transfect, finally allowing relevant gene expression experiments in almost all cell types (Fig. 1).

jetMESSENGER outperforms DNA competitor

Fig. 1. jetMESSENGER™ outperforms its main DNA transfection reagent competitor. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection of eGFP mRNA (5meC, pseudo-uridine, Trilink™) or plasmid DNA encoding for eGFP. Conditions were used according to the manufacturers’ recommendation.

Works on a variety of cell lines

With jetMESSENGER™, achieve outstanding transfection efficiency in a wide variety of cell lines, from adherent and suspension cancer cells, to sensitive primary cells and neurons. jetMESSENGER™ is also perfectly suitable for reprogramming experiments in fibroblasts and stem cells (Table 1).

jetMESSENGER - Table transfection efficiency
Table 1. jetMESSENGER™ leads to high transfection efficiency in a wide variety of cell types.

Easy to use

mRNA transfection is as easy as DNA transfection: just dilute the mRNA in the provided buffer, add jetMESSENGER™ to the diluted mRNA and add the mix to the cells’ growth medium in presence/absence of serum (compatible with antibiotics) (Fig. 2).

jetMESSENGER - Protocol
Fig. 2. Easy transfection process

Extremely gentle to cells

By eliminating the need to reach the nucleus for efficient expression, jetMESSENGER™ allows transfection of quiescent and slow dividing cells. In addition, jetMESSENGER™ operates through an extremely gentle process. Cell viability remains extremely high during transfection and cell morphology is maintained (Fig. 3). Make your transfection experiments physiologically relevant!

jetMESSENGER - Microscopy

Fig. 3. jetMESSENGER™ provides a better cell viability and a higher protein expression than DNA transfection. Primary Rat Cortical neurons, Hep G2 and mouse stem cells were analyzed 48 h after transfection using phase contrast and fluorescent microscopy. The transfections were performed with jetMESSENGER™/eGFP mRNA (5meC, pseudo-uridine, Trilink™) and L2K/eGFP plasmid DNA according to the manufacturers’ recommendations for each reagent.

mRNA transfection

jetMESSENGER™ is perfectly suited for gene expression in difficult to transfect cells such as neurons, primary cellsstem cells and various cancer cell lines, through mRNA transfection (Fig. 1 & Fig. 2).

jetMESSENGER - Cells

Fig. 1. jetMESSENGER™-mediated transfection in different hard to transfect cells. Various cell lines were analyzed by fluorescent microscopy 48 h after transfection. Transfections were performed with jetMESSENGER™/eGFP mRNA (5meC, pseudo-uridine, Trilink™) according to the manufacturer’s recommendation. Data on OVCAR-3, NCCIT, MDA-MB-231 & SW1353 cell lines are kindly provided by Inserm U1199 « BioTICLA » (Caen, France).

jetMESSENGER - Primary mouse lung endothelial cells

Fig. 2. jetMESSENGER™ is suitable to transfect mRNAs in primary mouse lung engothelial cells. Primary lung cells were analyzed by fluorescent microscopy 48 hours post transfection. Cotransfection was performed with modified RNAs for nuclear GFP and mCherry using jetMESSENGER™. Data are kindly provided by T. Itkin, Weill Cornell Medicine, USA.

Neurology applications

jetMESSENGER™ leads to high transfection efficiency in primary neurons, while maintaining a physiological and healthy cell morphology for several days, allowing long term expression for neurology applications (Fig. 3.).

 jetMESSENGER - Neuron brightfield JetMESSENGER - Neuron fluorescence

Fig. 3. jetMESSENGER™ is suitable to transfect mRNA in primary rat cortical neurons. Primary rat cortical neurons were analyzed by bright-field and fluorescent microscopy 48 h after transfection. Transfection was performed with jetMESSENGER™/eGFP mRNA (5meC, pseudo-uridine, Trilink™) according to the manufacturer’s recommendations.

CRISPR/Cas9 applications

jetMESSENGER™ is perfectly suited for RNA-based genome editing using CRISPR/Cas9 .

Cell reprogramming

Cells transfected with jetMESSENGER™ show a high viability and a long term expression of the protein of interest, making jetMESSENGER™ the ideal transfection reagent for reprogramming studies. It is now possible to turn any cell of the body into pluripotent stem cells. For example, it is possible to use mRNA transfection with jetMESSENGER™ to transform fibroblasts into IPs.

 jetMESSENGER - Fibroblast brightfield jetMESSENGER - Fibroblast fluorescence

Fig. 4. jetMESSENGER™ is suitable to transfect mRNA in BJ cells (human fibroblast). BJ cells were analyzed by bright-field and fluorescent microscopy 48 h after transfection. Transfection was performed with jetMESSENGER™/eGFP mRNA (5meC, pseudo-uridine, Trilink™) according to the manufacturer’s recommendations.

Immunotherapy applications

Efficient cancer and immune cells transfection with jetMESSENGER™ offers a wide range of cancer and immunotherapy applications.

“Application of jetMESSENGER reagent to introduce modified RNA molecules into primary lung mouse endothelial cells (a cell population which is generally hard to transfect with DNA/RNA using non-viral methodology) resulted with the highest transfection rate (>70%) and with the lowest toxicity effects as compared to other tested RNA/DNA transfection reagents (jetPEI, TransIT, & RNAiMAX).”
Itkin T., Weill Cornell Medicine, New York, United States

“jetMESSENGER appears as a very effective reagent for RNA transfection in human cancer cells of various origins. The expression level intensity and the transfected cells proportion were very impressive as compared to conventional DNA transfection reagents, without any sign of cytotoxicity.”
Brotin E., Abeilard E., Denoyelle C., Poulain L. Inserm U1199 « BioTICLA », Caen, France

“The protocol and handling of jetMESSENGER Kit was very simple and easy to use in comparison to other mRNA kits we tested. In our hands, jetMESSENGER is the only component that resulted in 60% transfection efficiency in peritoneal macrophages, in less than 9h while not affecting cell viability.”
Herb M., Universi
ty of Cologne, Germany

Here is a selection of relevant references using jetMESSENGER™, more are available in our Polyplus-transfection Database.

Viegas C.S.B., Costa R.M., Santos L., Videira P.A., Silva Z., Araújo N., Macedo A.L., Matos AP., Vermeer C., Simes D.C. (2017). Gla-rich protein function as an anti-inflammatory agent in monocytes/macrophages: Implications for calcification-related chronic inflammatory diseases. PLoS Ones, DOI:10.1371/journal.pone.0177829

Sultana N., Magadum A., Hadas Y., Kondrat J., Singh N., Youssef E., Calderon D., Chepurko E., Dubois N., Hajjar RJ., Zangi L. (2017). Optimizing Cardiac Delivery of Modified mRNA. Mol Ther, DOI:10.1016/j.ymthe.2017.03.016

Every batch of jetMESSENGER™ mRNA transfection reagent is tested in-house by mRNA transfection of CaCo-2 cells with a GFP-expressing mRNA and each vial of reagent is provided with a Certificate of Analysis.