in vivo-jetPEI®-Gal

Improved delivery to liver

  • Suitable for in vivo delivery of any nucleic acid
  • Very simple to use: two-step protocol
  • Safe: No inflammatory response triggered
  • Protocols tailored for your application by Polyplus-transfection delivery experts

Specifications

Reagent

in vivo-jetPEI®-Gal

Molecule delivered

DNA
siRNA
miRNA
oligonucleotides
mRNA

Applications

in vivo functional studies
Cancer therapy

Targeted organs

Improved delivery to liver

Injection routes

Hydrodynamic injection
Systemic delivery (Tail vein, intravenous, intraperitoneal injection)
Local injection

Number of transfections

100 µl of in vivo-jetPEI®-Gal delivery reagent is sufficient to perform 15 to 25 intravenous injections in mouse.

Storage

-20 °C, for at least 12 months

Provided with

10% glucose solution

Summary

in vivo-jetPEI® is a powerful polymer based reagent used to deliver any kind of nucleic acid to target a wide range of organs and tissues in animals. in vivo-jetPEI® ligand-conjugated derivatives have been designed to enhance delivery to cell expressing specific receptors.
in vivo-jetPEI®-Gal is a galactose-conjugated in vivo-jetPEI® designed to enhance delivery to cells expressing galactose-specific membrane lectins, such as hepatocytes bearing the asialoglycoprotein receptor (ASGP-R or Gal/GalNAc receptor).

Ordering information

Catalog NumberAmount of reagentAmount of 10% glucose solution
202-10G0.1 ml10 ml

Suitable for in vivo delivery of DNA, siRNA, miRNA, oligonucleotides and mRNA

in vivo-jetPEI®-Gal is the reagent of choice to deliver any type of nucleic acid to mediate gene expression or gene silencing in various tissues, with enhanced targeting to liver.
in vivo-jetPEI®-Gal is very versatile as it is suitable for the delivery of plasmid DNA, siRNA, shRNA, miRNA, oligonucleotides and mRNA.

Easy two-step protocol

in vivo-jetPEI®-Gal is a very easy-to-use delivery reagent. The protocol consists in preparing the nucleic acid and reagent separately in 5% glucose solution, mixing them together and then, after a 15 min incubation time at room temperature, injecting the nucleic acid/ in vivo-jetPEI®-Gal complexes formed into animal (Fig. 1 ).

<em>in vivo</em>-jetPEI-Gal - Protocol
Fig. 1: in vivo-jetPEI-Gal – Protocol

Suitable for any animal model

in vivo-jetPEI®-Gal is ideal for nucleic acid delivery in mice. However, the protocol is so easy and versatile that it can be adapted to other species including rat.
Our delivery experts are available to adapt our protocol to your animal model and provide you with starting conditions for your application (Please contact the technical support team at support@polyplus-transfection.com).

Safe: no inflammatory response induced

in vivo-jetPEI®-Gal offers a reliable and safe alternative to viral vectors as it does not induce any significant inflammatory response.

in vivo functional studies

in vivo-jetPEI®-Gal is perfectly suited to study gene function in the liver in vivo and provides the easiest method for the validation into animals of in vitro functional studies.

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Cancer Therapy

in vivo-jetPEI®-Gal-based nucleic acid delivery can be used for tumor growth inhibition studies in the liver.

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Here is a selection of relevant references using in vivo-jetPEI®-Gal, more are available in our Polyplus-transfection Database.

Nickerson, H. D., Colledge, W. H. (2004). A LacZ-based transgenic mouse for detection of somatic gene repair events in vivo., Gene Ther 11, 1351.

Xia, Y. J., Zeng, D., Xia, L. M., Yu, F., Lin, H. H., Zhou, L., Tian, D. A., Liu, M. (2012). Role of monokine induced by interferon-gamma in liver injury induced by hepatitis B virus in mice., J Viral Hepat 19, 509-1.

Polyplus-transfection® is ISO 9001 Quality Management System accredited since 2002; this level of certification assures global customers that the supplier has established reliable and effective processes for product development, manufacturing, sales and customer support.

Each lot of in vivo-jetPEI®-Gal is validated by DNA (pCMV-Luciferase) delivery into mouse through intravenous injection. The amount of luciferase (ng) per mg of protein is indicated on the certificate of analysis for each lot of reagent. Moreover, absence of endotoxin is verified in every lot of in vivo-jetPEI®-Gal.