Publications from the Polyplus Team
Nothisen, M., Kotera, M., Voirin, E., Remy, JS. and Behr JP. (2009) Cationic siRNAs provide Carrier-free gene silencing in animal cells. J. Am. Chem. Soc., 131, 17730-17731.
Paris, C., Moreau, V., Deglane, G., Voirin, E., Erbacher, P. and Lenne-Samuel, N. (2010) Zip nucleic acids are potent hydrolysis probes for quantitative PCR. Nucl. Acids Res., doi: 10.1093/nar/gkp1218. Download the PDF
Nothisen, M., Kotera, M., Voirin, E., Remy, JS. and Behr JP. (2009) Cationic siRNAs provide Carrier-free gene silencing in animal cells. J. Am. Chem. Soc., 131, 17730-17731.
Moreau, V., Voirin, E., Paris, C., Kotera, M., Nothisen, M., Remy, J-S., Behr, J-P., Erbacher, P. and Lenne-Samuel, N.,. (2009). Zip nucleic acids (ZNAs): new high affinity oligonucleotides as potent primers for PCR and reverse transcription. Nucl. Acids Res., 37: e130; doi:10.1093/nar/gkp661. Download the PDF
Noir, R., Kotera, M., Pons, B., Remy, JS., and Behr, JP. (2008) Oligonucleotide-oligospermine conjugates (Zip Nucleic Acids): a convenient means of finely tuning hybridization temperatures. J Am Chem Soc, 130, 13500-13505.
Voirin, E., Behr, JP. and Kotera M. (2007) Versatile synthesis of oligodeoxyribonucleotide-oligospermine conjugates. Nat Protoc, 2, 1360-1367.
Bonnet, M. E., Erbacher, P., and Bolcato-Bellemin, A. L. (2008). Systemic Delivery of DNA or siRNA Mediated by Linear Polyethylenimine (L-PEI) Does Not Induce an Inflammatory Response. Pharm Res, (12):2972-82
Bolcato-Bellemin, A. L., Bonnet, M. E., Creusat, G., Erbacher, P., and Behr, J. P. (2007). Sticky overhangs enhance siRNA-mediated gene silencing. Proc Natl Acad Sci U S A 104, 16050-16055.
Hassani, Z., Lemkine, G. F., Erbacher, P., Palmier, K., Alfama, G., Giovannangeli, C., Behr, J. P., and Demeneix, B. A. (2005). Lipid-mediated siRNA delivery down-regulates exogenous gene expression in the mouse brain at picomolar levels. J Gene Med 7, 198-207.
Louis, M. H., Dutoit, S., Denoux, Y., Erbacher, P., Deslandes, E., Behr, J. P., Gauduchon, P., and Poulain, L. (2006). Intraperitoneal linear polyethylenimine (L-PEI)-mediated gene delivery to ovarian carcinoma nodes in mice. Cancer Gene Ther 13, 367-374.
Lisziewicz, J., Trocio, J., Whitman, L., Varga, G., Xu, J., Bakare, N., Erbacher, P., Fox, C., Woodward, R., Markham, P., et al. (2005). DermaVir: a novel topical vaccine for HIV/AIDS. J Invest Dermatol 124, 160-169
Arnold, A. S., Laporte, V., Dumont, S., Appert-Collin, A., Erbacher, P., Coupin, G., Levy, R., Poindron, P., and Gies, J. P. (2006). Comparing reagents for efficient transfection of human primary myoblasts: FuGENE 6, Effectene and ExGen 500. Fundam Clin Pharmacol 20, 81-89.
Mennesson, E., Erbacher, P., Kuzak, M., Kieda, C., Midoux, P., and Pichon, C. (2006). DNA/cationic polymer complex attachment on a human vascular endothelial cell monolayer exposed to a steady laminar flow. J Control Release 114, 389-397.
Mennesson, E., Erbacher, P., Piller, V., Kieda, C., Midoux, P., and Pichon, C. (2005). Transfection efficiency and uptake process of polyplexes in human lung endothelial cells: a comparative study in non-polarized and polarized cells. J Gene Med 7, 729-738.
Ndoye, A., Dolivet, G., Hogset, A., Leroux, A., Fifre, A., Erbacher, P., Berg, K., Behr, J. P., Guillemin, F., and Merlin, J. L. (2006). Eradication of p53-mutated head and neck squamous cell carcinoma xenografts using nonviral p53 gene therapy and photochemical internalization. Mol Ther 13, 1156-1162.
Ndoye, A., Bouali, S., Dolivet, G., Leroux, A., Erbacher, P., Behr, J. P., Berg, K., Guillemin, F., and Merlin, J. L. (2004a). Sustained gene transfer and enhanced cell death following glucosylated-PEI-mediated p53 gene transfer with photochemical internalisation in p53-mutated head and neck carcinoma cells. Int J Oncol 25, 1575-1581.
Ndoye, A., Merlin, J. L., Leroux, A., Dolivet, G., Erbacher, P., Behr, J. P., Berg, K., and Guillemin, F. (2004b). Enhanced gene transfer and cell death following p53 gene transfer using photochemical internalisation of glucosylated PEI-DNA complexes. J Gene Med 6, 884-894.
Dolivet, G., Merlin, J. L., Barberi-Heyob, M., Ramacci, C., Erbacher, P., Parache, R. M., Behr, J. P., and Guillemin, F. (2002). In vivo growth inhibitory effect of iterative wild-type p53 gene transfer in human head and neck carcinoma xenografts using glucosylated polyethylenimine nonviral vector. Cancer Gene Ther 9, 708-714.
Dobbs, W., Heinrich, B., Bourgogne, C., Donnio, B., Terazzi, E., Bonnet, M-E., Stock, F., Erbacher, P., Bolcato-Bellemin, A-L., and Douce, L., (2009). Mesomorphic imidazolium salts: new vectors for efficient siRNA transfection. JACS in press.
Pons, B., Mouhoubi, L., Adib, A., Godzina, P., Behr, J. P., and Zuber, G. (2006). Omega-Hydrazino linear polyethylenimine: a monoconjugation building block for nucleic acid delivery. Chembiochem 7, 303-309.
Zhou, J., Wu, J., Hafdi, N., Behr, J. P., Erbacher, P., and Peng, L. (2006). PAMAM dendrimers for efficient siRNA delivery and potent gene silencing. Chem Commun (Camb), 2362-2364.
Erbacher, P., Bettinger, T., Brion, E., Coll, J. L., Plank, C., Behr, J. P., and Remy, J. S. (2004). Genuine DNA/polyethylenimine (PEI) complexes improve transfection properties and cell survival. J Drug Target 12, 223-236.
Zaric, V., Weltin, D., Erbacher, P., Remy, J. S., Behr, J. P., and Stephan, D. (2004). Effective polyethylenimine-mediated gene transfer into human endothelial cells. J Gene Med 6, 176-184
Weill, C., Biri, S., and Erbacher, P. (2008b). Cationic lipid-mediated intracellular delivery of antibodies into live cells. Biotechniques 44, Pvii-Pxi.7
Weill, C., Biri, S., Adib, A., and Erbacher, P. (2008a). A practical approach for intracellular protein delivery. Cytotechnology 56, 41-48.
Williamson, J.F. (2008). Advances and perspectives in intracellular biomolecule delivery. Pharmaceutical Technology Europe, July 2008, p. 43-46.
Zuber, G., Dauty, E., Nothisen, M., Belguise, P., and Behr, J. P. (2001). Towards synthetic viruses. Advanced Drug Delivery Reviews 52, 245-253


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