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Bioproduction: Transfection in Regular Media

  • High efficacy on a range of cells such as CHO and HEK293
  • Used in bioreactors, bags and spinner flask
  • Batch to batch reproducibility guaranteed
  • Free of animal origin component
  • Fully licensed PEI for Transfection
  • Available in bulk (1- 100L)
  • Qualified for cGMP virus manufacturing and protein production
  • Large scale protocols and technical support available

jetPEI™, a fully synthetic chemically-defined reagent of high quality grade matches perfectly all stringent biomanufacturing requirements providing a robust and fully licensed transfection reagent for recombinant protein production.

INTELLECTUAL PROPERTY
The use of polyethylenimine (PEI) or polypropylenimine (PPI) or cationic polymers similar in structure thereto for transfecting cells, as well as compositions comprising these cationic polymers and at least one nucleic acid, are the subject matter of U.S. Patent No. 6,013,240, EP Patent No. 0770140 and foreign equivalents, for which Polyplus-transfection™ is the worldwide exclusive licensee.

More information:

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Catalog Number Amount of reagent Amount of NaCl solution
101-01N 0.1 ml 5 ml
101-10N 1 ml 50 ml
101-10 1 ml -
101-40N 4 x 1 ml 4 x 50 ml
101-40 4 x 1 ml -
101B-010N 10 mL 2 x 250 mL
101B-10 10 mL -
One ml of jetPEI™ is sufficient to perform 500 transfections in 24-well plates.Large pack sizes available upon request (50 mL, 100 mL and 1L). Please contact us.

 

For large scale production of recombinant proteins such as human therapeutic molecules including proteins, monoclonal antibodies, vaccine antigens and viruses  (rAAV, r-Adenoviruses and r-Lentivirus) by transient or stable transfection, jetPEITM is  the reagent of choice (Fig. 1).

Fig 1: One liter and one ml of jetPEI&trade
Figure 1: One liter and one ml of jetPEI™

By ensuring a robust, high quality DNA transfection, it leads to efficient and sustained protein expression levels and most importantly guarantees batch to batch reproducibility.

 

With a department dedicated to qualified reagents, Polyplus is able to supply any reagent grade you may require. Furthermore, the QC provided can be tailored to suit any protein production laboratory.

 

Polyplus-transfection provides prime and personalized service including scientific support to suit the pharmaceutical industry requirements.

If you wish to obtain the protocols for 1L of culture or more please contact the Polyplus scientific support specialists.

 

Polyplus-transfection jetPEI™ is also well-suited for routine virus and protein production in Flasks. For example, it is recommended by Corning for transfection of CHO and HeLa cells in HYPERflasks®.

 

Polyplus-transfection is the worldwide exclusive licensee of PEI (Polyethylenimine) and derivates for stable and transient transfection of cells.
The purchase of jetPEI™ conveys the buyer right to perform transfection using jetPEI in biomanufacturing applications.

« The use of polyethylenimine (PEI) or polypropylenimine (PPI) or cationic polymers similar in structure thereto for transfecting cells, as well as compositions comprising these cationic polymers and at least one nucleic acid, are the subject matter of U.S. Patent No. 6,013,240, EP Patent No. 0770140 and foreign equivalents, for which Polyplus-transfection™ is the worldwide exclusive licensee. »

For more information, see Licenses.