Polyplus Transfection

Welcome to Polyplus-transfection

Polyplus-transfection is focused on developing reagents for intracellular delivery of biomolecules. Its expertise includes in vitro and in vivo delivery of genes, oligonucleotides, siRNA and proteins.

> In vivo delivery: in vivo-jetPEI™
For gene delivery applications, pre-clinical studies & RNA interference, multiple adminstration routes in many species

> DNA transfection: jetPEI™
Extremely efficient, easy to use: compatible with serum

> siRNA transfection: INTERFERin™
Over 90% gene silencing at 1 nM siRNA in a wide variety of cells

> Protein/virus production: Fecturin™
Versatile: efficient in both HEK 293 & CHO synthetic media

> Protein/antibody intracellular delivery: PULSin™
Effective and robust delivery into the cytoplasm, easy to use

> Cell-specific DNA transfection reagents:

Insect cells: FectoFly™ for baculovirus production
Primary endothelial cells: jetPEI™-HUVEC
Macrophage-like cells: jetPEI™-Macrophage
Hepatocyte cells: jetPEI™-Hepatocyte
Epithelial and endothelial cells: jetPEI™-RGD

Novel sticky siRNA design to enhance RNAi
In order to improve siRNA delivery in vivo, Polyplus-transfection has recently developed a novel design of siRNA. By including longer overhangs, we have generated sticky siRNA (ssiRNA) that are able to form concatemers in the presence of in vivo-jetPEI™, thereby mimicking the structure of DNA and thus enhancing siRNA delivery (Bolcato-Bellemin (2007) PNAS 104:16050).

Meet the Polyplus team at:
> ASGT 2008 Boston USA (28 May – 1 June), The Annual Meeting of the American Society for Gene Therapy , Booth #416
> NSTI Nanotech 2008 Boston, USA (1-5 June), Merck Innovation Symposium

The Polyplus in vivo Consortium is a network of scientists and clinicians created to facilitate the development of gene therapy applications using non-viral delivery tools. Join the new Polyplus in vivo Consortium!

> Request or download the 2008 catalog!
> Request or download Polyplus News!