Protocolin vivo-jetPEI™ is broadly used as an in vivo delivery reagent. However, for specific applications such as siRNA delivery to the brain, Polyplus-transfection has developed jetSI™ 10 mM.
The choice of the most effective siRNA carrier may depend on the in vivo context. Indeed, while in vivo-jetPEI™ was superior to cationic liposomes for plasmid DNA delivery in the mouse brain, jetSI™ 10 mM was found to be the carrier of choice for siRNA delivery to this organ (Froidevaux et al. (2006), EMBO Rep 7:1035; Guissouma et al. (2006), Neurosci Lett 406: 240; Kumar et al. (2006), PLOS Med. 3: 0505; Hassani et al. (2005), J Gene Med 7:198).
When using plasmid based approaches (shRNA) in the brain, we recommend in vivo-jetPEI™.
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Please note that neither glucose solution nor DOPE are included. |
1. Hassani, Z., Lemkine, G.F., Erbacher, P., Palmier, K., Alfama, G., Giovannangeli, C., Behr, J.P. and Demeneix, B.A. (2005) Lipid-mediated siRNA delivery down-regulates exogenous gene expression in the mouse brain at picomolar levels. J Gene Med 7(2): 198-207.
2. Froidevaux M. S., Berg P., Seugnet I., Decherf S., Becker N., Sachs L. M., Bilesimo P., Nygard M., Pongratz I., Demeneix B. A., (2006) The co-chaperone XAP2 is required for activation of hypothalamic thyrotropin-releasing hormone transcription in vivo EMBO Rep (7) 1182.
3. Guissouma H., Froidevaux M. S., Hassani Z., Demeneix B. A., (2006) In vivo siRNA delivery to the mouse hypothalamus confirms distinct roles of TR beta isoforms in regulating TRH transcription Neurosci Lett (406) 240-3.
4. Kumar P., Lee S. K., Shankar P. and Manjunath N. (2006) A single siRNA suppresses fatal encephalllitis induced by two different flaviviruses PLOS Med 3: 0505-1.
