- High protein expression and baculovirus production
- Global transfection solution for insect cells
- High transfection efficiency in adherent and suspension insect cells
- Guaranteed free of material from animal origin
FectoFly® is a DNA transfection reagent dedicated to insect cells for high level production of baculovirus and recombinant proteins.
FectoFly® is also well-suited for high level transgene expression in insect cells.
|Fig 1. Protein production in S2 cells over 5 days using FectoFly® transfection reagent. A suspension culture of 106 cells/ml was transfected using 2 µg pCMV-EGFPLuc and 6.4 µl of FectoFly® per ml of culture in Insect-XPRESS™ medium (Lonza).|
Figure 2. Comparison of FectoFly® in various adherent cell lines. Sf9, Sf21, S2 or Tn5 adherent cells were transfected in 24-well plates pCMV-EGFP-Luc plasmid. Luciferase assays were performed 72 h after transfection.
|Fig. 2. Comparison of FectoFly® in various adherent cell lines. Sf9, Sf21, S2 or Tn5 adherent cells were transfected in 24-well plates pCMV-EGFP-Luc plasmid. Luciferase assays were performed 72 h after transfection.|
FectoFly® allows successful transfection of adherent and suspension insect cell cultures both in the latest generation of syntheticserum-free media (Fig.1, 3 – Sf21 and Sf9) and in the presence of serum (Fig.3 – S2). Transfection efficiency ranges routinely from 30% to 50% and cell viability is above 80%.
|Fig 3. GFP expression in Sf21, Sf9 cells grown in suspension, 72 h after transfection of pCMV-EGFP-Luc plasmid using FectoFly®.|
FectoFly® was compared to other commercially available reagents dedicated to the transfection of insect cells (Fig. 4). As shown for Sf9 cells, the highest protein expression levels were obtained with FectoFly®.
|Fig 4. Comparison of FectoFly® with other insect cell-specific transfection reagents. Sf9 adherent cells were transfected in 6-well plates with pCMV-EGFPLuc plasmid using insect cell transfection reagents according to the supplier’s recommendations.|
|Transfection of insect cells with FectoFly® is straightforward.
o Fast : 3 steps protocol
o Simple: 1 µl of FectoFly® per µg of DNA, 1:1 ratio
o Versatile: efficient in serum-free synthetic media and in the presence of serum