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Virus Classical Media: jetPRIME®

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  • High viral titers
  • Suitable for multiple plasmid cotransfection
  • Requires small amounts of DNA
  • Perfectly suited for HEK-293T and VERO cells
  • Compatible with both serum and antibiotics

jetPRIME® transfection reagent is highly effective for routine virus production in adherent cells grown in classical media such as DMEM in presence of serum.

More information:

Expand All Tabs
Catalog Number Amount of reagent Amount of buffer
114-01 0.1 ml 5 ml
114-07 0.75 ml 60 ml
114-15 1.5 ml 2 x 60 ml
114-75 5 x 1.5 ml 10 x 60 ml
114-75C 5 x 1.5 ml 120 ml x 5x conc.

1.5 ml is sufficient to perform ca.375 transfections in 6-well plates. Bulk quantities are available upon request.

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jetPRIME® gives high viral titers for both AAV and lentivirus production.

 

Virus production often requires the simultaneous cotransfection of multiple plasmids to form viral particles. With its ability to efficiently compact several DNA molecules into positively charged transfection complexes, jetPRIME® is perfectly suited for multiple plasmid cotransfection.

 

jetPRIME® is highly efficient and only requires small amount of DNA for high transfection efficiency in adherent cells commonly used for virus production. More information on the main jetPRIME® page.

 

Transfection efficiency using jetPRIME® reaches up to 90% in cells commonly used for virus production, such as HEK-293 and derivatives, VERO, CHO and BHK cells. Hence, jetPRIME® is the reagent for choice for viral expression vectors transfection, leading to high viral titers.

 

The protocol for jetPRIME® is easy to use since it is compatible with the use of both serum and antibiotics avoiding the need for media changes and facilitating the manipulation of a large number of vessels.

Protocol for virus production using jetPRIME Fig. 1. Easy jetPRIME® protocol for virus production.

 

Transfection efficiency using jetPRIME® reaches up to 90% in cells commonly used for virus production, such as HEK-293 and derivatives, CHO, VERO and BHK cells. Hence, jetPRIME® is the reagent for choice for viral expression vectors transfection, leading to high viral titers.