- Fast and efficient methods to transfect cells for HTS
- Exceptionally reproducible results
- Well-suited for automated approaches
- Compatible with serum and antibiotics
- Reverse, batch & forward protocols available
jetPEI® transfection reagent is a linear polyethylenimine derivative, free of components of animal origin, providing highly effective and reproducible gene delivery.
jetPEI® transfection reagent is therefore particularly well suited for automated or manual HTS (High Throughput Screening) with three protocols available: reverse, batch and forward.
NaCl complex-formation solution included (adapted to proper complex-formation, as indicated in the protocol).
One ml of jetPEI® is sufficient to perform 2 000 transfections in 96-well plates.
The reverse protocol is the most appropriate when transfecting a pool of genes, such as a DNA library (Fig. 1). In this protocol, the jetPEI®/DNA complexes are prepared or deposited in the wells prior to addition of the cells. Complexes are stable for up to 4 hours (Fig. 2).
The batch protocol was developed to prepare a homogeneous pool of transfected cells. For this purpose, the cells are transfected just after trypsinization, while still in suspension. This protocol is prefered for drug screening applications and allows rapid processing, one day faster than the forward protocol.
In the forward protocol, the cells are split the day before transfection and the jetPEI®/DNA complexes are added to the adherent cells.
|Figure 1. jetPEI® reverse transfection protocol for HTS application.|
Complexes formed with the water-soluble polymer jetPEI® and DNA allow efficient transfection for up to 4 hours, in contrast to lipid-based reagents and calcium phosphate. Thus they allow plenty of time to dispense the complexes into the plates (Fig. 2).
HTS DNA transfection using jetPEI® gives highly consistent transfection efficiency from batch-to-batch (Fig. 3).
|Figure 3. Batch-to-batch reproducibility using jetPEI®. For each lot, HeLa cells were transfected in triplicate in the presence of serum in triplicate using the standard protocol in 24-well plate.|
jetPEI® successfully delivers genes to various adherent and non-adherent cell lines, as well as primary cells (Table 1). Over 550 publications using jetPEI® can be found in the Product Citation Database on the Polyplus website. In addition, a Cell Transfection Database gives specific transfection conditions for over 400 cell lines and primary cells.
|Table 1. Some common cell lines and primary cells successfully transfected using jetPEI®.|
jetPEI® was compared to several other popular transfection reagents (Fig. 4). jetPEI® was found to offer the best performance: high efficiency and low variability (small standard deviation).